This cell line has not been specifically tested for XMRV. However, literature indicates that the XMRV-contaminated LNCaP cell line originated from the Cleveland Clinic as the result of laboratory cross-contamination. The LNCaP cell line originated in 1977 (Horoszewicz) and was deposited with ATCC in 1989. The ATCC line has never tested positive for XMRV. These cells are supplied by ECACC, who acquired the line from ATCC. It is unlikely that this line is XMRV-positive.
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Size/SKU | Availability | Price |
|---|
About This Item
UNSPSC Code:
41106514
Biological source:
human prostate (carcinoma)
Relevant disease(s):
metastasis
Growth mode:
Adherent
Karyotype:
Pseudodiploid male; seven marker chromosomes, modal number 46, range 33 to 91
Morphology:
Epithelial-like
Manufacturer/tradename:
ECACC
Pricing and availability is not currently available.
biological source
human prostate (carcinoma)
manufacturer/tradename
ECACC
packaging
tube of 5 μg 89110211-DNA-5UG, pkg of vial of cells 89110211-1VL
growth mode
Adherent
karyotype
Pseudodiploid male; seven marker chromosomes, modal number 46, range 33 to 91
morphology
Epithelial-like
products
Prostate acid phosphatase, prostate specific antigen
receptors
Androgen, estrogen
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
metastasis
shipped in
dry ice
storage temp.
−196°C
Application
LNCap Clone FGC Cell Line has been used:
- to study the effect of sex steroid hormones in regulating the expression of oligoadenylate synthetase 1 (OAS1)
- to study the role of curcumin and resveratrol in redox control and ability to promote apoptosis
- to investigate low molecular weight thiols present in the deproteinized extract of the cell line after derivatization
Biochem/physiol Actions
Derived from a metastasis at the left supraclavicular lymph node of a 50 year old patient with a confirmed diagnosis of metastatic prostate carcinoma. Growth and acid phosphatase production is affected by 5-alpha-dihydrotestosterone. They do not form a uniform monolayer and attach only lightly to the substrate. When shipped, cells detach from flask and can either be incubated 24-48 hours to allow attachment or be collected by centrifugation (150xg, 15 minutes) and reseeded.
Human Caucasian prostate carcinoma
Preparation Note
RPMI 1640 + 2mM Glutamine + 1.0 mM sodium pyruvate + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.i.e. seeding at 1-3 x 10,000 cells/cm2 Cells grow slowly in clusters and can be disaggregated by repeated pipetting. After subculture cells may take up to 48 hours to reattach. Do not disturb during this period. Medium is rapidly acidified, therefore change twice per week.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
1 of 1
This Item | |||
|---|---|---|---|
| description NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 89110211, human prostate (carcinoma), Epithelial-like | description NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 6020201, human prostate (metastasis), Epithelial | description NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, human prostate, Epithelial | description NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA. |
| packaging tube of 5 μg 89110211-DNA-5UG, pkg of vial of cells 89110211-1VL | packaging tube of 5 μg 06020201-DNA-5UG, pkg of vial of cells 06020201-1VL | packaging pkg of vial of cells 10112303-1VL, tube of 5 μg 10112303-DNA-5UG | packaging tube of 5 μg 94101454-DNA-5UG, pkg of vial of cells 94101454-1VL |
| biological source human prostate (carcinoma) | biological source human prostate (metastasis) | biological source human prostate | biological source rat prostate |
| morphology Epithelial-like | morphology Epithelial | morphology Epithelial | morphology Not specified |
| storage temp. −196°C | storage temp. −196°C | storage temp. - | storage temp. −196°C |
| receptors Androgen, estrogen | receptors Androgen receptor | receptors Not specified | receptors Not specified |
| karyotype Pseudodiploid male; seven marker chromosomes, modal number 46, range 33 to 91 | karyotype Refer to reference (Korenchuk et al., 2001) | karyotype Not specified | karyotype Modal no. 64 |
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
-
Does the cell line LNCaP Clone FGC contain contaminating XMRV, like the parental LNCaP cell line (Sakuma, T., et al. (2014). Biochemical and Biophysical Research Communications, 447(1), 216-220)?
1 answer-
Helpful?
-
-
Hi,I we are interested to purchase LNCap Clone FGC Cell Line human. My question is are they alos expressed PSMA?
1 answer-
This cell line is a product of ECACC cell line. These cells are not tested for PSMA expression. However, there are a number of publications that suggest that prostate-specific membrane antigen is expressed. Please see the links below to review several examples:
https://journals.lww.com/cancerjournal/fulltext/2014/10010/how_prostate_specific_membrane_antigen_level_may.25.aspx
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8650835/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6214026/Please see the link below to review this cell line profile at Cellosaurus:
https://www.cellosaurus.org/CVCL_1379Helpful?
-
-
How do I handle Cell Line frozen cells upon arrival?
1 answer-
Upon receipt, frozen ampules should be transferred directly to vapor phase liquid nitrogen without delay (-135°C) or liquid phase liquid nitrogen if vapor phase is not available. DO NOT use a -80°C freezer as an alternative; this will result in loss of viability.
Helpful?
-
-
What medium should I use when I thaw the Cell Line cells?
1 answer-
The medium for each cell line is listed on the product page. It is specific to each cell line. If not visible on the Sigma-Aldrich website product page, the HPA website also contains the same information (www.hpacultures.org.uk). The product number for the cell line is the same on either site.
Helpful?
-
-
What passage are my Cell Line cells?
1 answer-
If the passage of the cells is known, it is listed on the product page at the HPA website (www.hpacultures.org.uk).
Helpful?
-
-
Why is Vapor Phase Liquid Nitrogen preferred for storage of Cell Line cells?
1 answer-
If ampules are immersed into liquid phase of liquid nitrogen, it increases the risk of the liquid seeping into the vial. This could lead to problems of cross-contamination and increased risk of the ampule exploding when thawed.
Helpful?
-
-
Are the Cell Line cells tested for viral pathogens?
1 answer-
HPA Cultures does not perform any viral testing on the cell lines.
Helpful?
-
-
How many Cell Line cells are in the vial?
1 answer-
Each vial of cells contains 2-3 x 106 cells in 1 ml of freezing media. This is in a 1.8 mL ampule. For exceptionally large cells, counts may decrease. Suspension cells, generally smaller cells, may contain as many as 4-5 x 106 cells/vial to assure optimal viability upon thaw.
Helpful?
-
-
Are my Cell Line cells mycoplasma tested?
1 answer-
ECACC routinely tests all manufactured cell banks for mycoplasma.
Helpful?
-