C7527

Choline chloride

BioReagent, suitable for cell culture, suitable for insect cell culture, ≥98%

• Synonym(s): (2-Hydroxyethyl)trimethylammonium chloride
• Linear Formula: (CH₃)₃N(Cl)CH₂CH₂OH
• CAS Number: 67-48-1
• Molecular Weight: 139.62
• Beilstein: 3563126
• EC Number: 200-655-4
• MDL number: MFCD00011721
• UNSPSC Code: 12352205
• PubChem Substance ID: 24892968
• NACRES: NA.75

Properties

• biological source: synthetic (organic)
• product line: BioReagent
• Assay: ≥98%
• form: crystals
• mol wt: M_w 139.62 g/mol
• technique(s): cell culture | insect: suitable; cell culture | mammalian: suitable; cell culture | plant: suitable
• color: white
• pH: 5.0-6.5 (25 °C, 140 g/L)
• mp: 302-305 °C (dec.) (lit.)
• solubility: H₂O: 50 mg/mL
• SMILES string: [Cl-].C[N+](C)(C)CCO
• InChI: 1S/C5H14NO.ClH/c1-6(2,3)4-5-7;/h7H,4-5H2,1-3H3;1H/q+1;/p-1
• InChI key: SGMZJAMFUVOLNK-UHFFFAOYSA-M

Description

General description

Choline chloride is a quaternary ammonium salt and associates with hydrogen bond donors (HBD). It is essential for the vital physiological functions and is used as a nutritional additive. Choline is a source of methyl groups for methyl-transfer reactions.

Application

Choline chloride has been used:

• as a component of choline acetyltransferase (ChAT) buffer and for choline uptake in cultured human neuroblastoma (SK-N-SH) cells
• for choline standard curve generation for quantifying phospholipase D (PLD) activity
• as a component of slice solution for the dissection of entorhinal-hippocampal brain slices

Substrates

Choline acetyltransferase substrate

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves