C4733

BID, Caspase-8-cleaved from mouse

≥95% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous solution

• UNSPSC Code: 12352202
• NACRES: NA.32
• MDL number: MFCD03456214

Properties

• recombinant: expressed in E. coli
• Quality Segment: 200
• assay: ≥95% (SDS-PAGE)
• form: buffered aqueous solution
• UniProt accession no.: P70444
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: mouse ... Bid(12122)

Description

General description

Mouse BID cleaved with caspase-8 generates the amino-terminal fragment (amino acids 1-59, 7 kDa) and the carboxy-terminal fragment (amino acids 60-195, 15 kDa). On size exclusion chromatography cleaved BID elutes at 27 kDa indicating that the fragments remain associated.

Biochem/physiol Actions

Caspase-8-cleaved BID relocates from the cytosol to the outer mitochondrial membrane where its interaction with Bak alters mitochondrial membrane permeability.

Physical form

0.2 μm filtered solution in 25 mM HEPES, pH 7.5, and 0.1 M KCl.

Analysis Note

Measured by its ability to induce cytochrome c release from isolated mouse liver mitochondria.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable