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Key Documents

AV01005

Sigma-Aldrich

Anti-BCL2 antibody produced in rabbit

affinity isolated antibody

Synonym(s):

Anti-B-cell CLL/lymphoma 2

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

26 kDa

species reactivity

human

concentration

0.5 mg - 1 mg/mL

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... BCL2(596)

General description

BCL2 is an apoptotic inhibitor that suppresses lipid peroxidation. Rabbit Anti-BCL2 antibody binds to human BCL2.

Immunogen

Synthetic peptide directed towards the N terminal region of human BCL2

Application

Rabbit Anti-BCL2 antibody can be used for immunohistochemistry (4-8μg/ml, using paraffin embedded tissues) and western blot (1.0μg/ml) assays.
Western blot analysis was performed on NK-cell lymphoma and Leukemia cell lysates using rabbit anti-BCL2 antibody.

Sequence

Synthetic peptide located within the following region: GHTPHPAASRDPVARTSPLQTPAAPGAAAGPALSPVPPVVHLTLRQAGDD

Physical form

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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D M Hockenbery et al.
Cell, 75(2), 241-251 (1993-10-22)
Bcl-2 inhibits most types of apoptotic cell death, implying a common mechanism of lethality. Bcl-2 is localized to intracellular sites of oxygen free radical generation including mitochondria, endoplasmic reticula, and nuclear membranes. Antioxidants that scavenge peroxides, N-acetylcysteine and glutathione peroxidase

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