A6103

Aprotinin

recombinant, expressed in Nicotiana (tobacco), ≥5 TIU/mg protein, powder, ≥98% (SDS-PAGE), suitable for inhibition assay

• Synonym(s): BPTI, Basic pancreatic trypsin inhibitor, Kallikrein-trypsin inactivator, Kunitz protease inhibitor
• EC Number: 232-994-9
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• product name: Aprotinin Nicotiana tobacco, >= 5TIU/mg protein, >= 98 % SDS-PAGE | 9087-70-1, recombinant, expressed in Nicotiana (tobacco), ≥5 TIU/mg protein, ≥98% (SDS-PAGE)
• recombinant: expressed in Nicotiana (tobacco)
• Quality Level: 300
• description: recombinant form of thenative bovine-sequence aprotinin purified from plant tissue (tobacco)
• product line: BioUltra
• Assay: ≥98% (SDS-PAGE)
• form: powder
• specific activity: ≥5 TIU/mg protein
• mol wt: ~_6.5 kDa
• technique(s): inhibition assay: suitable
• solubility: 0.85% sodium chloride: 5 mg/mL
• UniProt accession no.: P00974
• storage temp.: 2-8°C
• Gene Information: cow ... PTI(404172)

Description

Unit Definition

One trypsin inhibitor unit (TIU) will decrease the activity of 2 trypsin units by 50% where one trypsin unit will hydrolyze 1.0 μmole of N-α-benzoyl-DL-arginine p-nitroanilide (BAPNA) per min at pH 7.8 at 25 °C.

Preparation Note

Contains no animal-derived components or impurities, and is manufactured by transient expression of the aprotinin message in RNA (+)-strand tobacco mosaic virus vectors propagated in non-transgenic Nicotiana plants. This is a recombinant form of the native, bovine-sequence aprotinin, which is traditionally isolated from bovine lung by methods involving fractional precipitation, gel filtration, and ion exchange chromatography. Unlike animal-derived aprotinin, this product is isolated and purified from plant tissue by proprietary methods.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves