This compound is relatively stable in powder form and ships at ambient temperatures globally, year round. Under these shipping conditions, transit times of up to 2 weeks are permitted. Brief temperature excursions are not expected to impact the quality of the product. However, extreme or long term temperature excursion studies have not been conducted. In the event of a temperature excursion, document the maximum temperature and duration, return the material to 2–8 °C, verify that the appearance remains white to light beige without caking or discoloration, and protect the product from light, and moisture. Please see the link below to access a Specification sheet. https://www.sigmaaldrich.com/specification-sheet/SIGMA/87748
87748
3,3′,5,5′-Tetramethylbenzidine
TMB substrate, chromogenic, ≥98.0% (NT), powder
Synonym(s):
BM blue, Sure Blue TMB, TMB, TMB Blotting Plus, TMB substrate
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About This Item
Linear Formula:
[-C6H2(CH3)2-4-NH2]2
CAS Number:
Molecular Weight:
240.34
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
259-364-6
MDL number:
Beilstein/REAXYS Number:
2808541
Pricing and availability is not currently available.
Product Name
3,3′,5,5′-Tetramethylbenzidine, ≥98.0% (NT)
Quality Segment
assay
≥98.0% (NT)
form
powder
technique(s)
UV/Vis spectroscopy: suitable
mp
166-171 °C, 168-171 °C (lit.)
storage temp.
2-8°C
SMILES string
Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2
InChI
1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3
InChI key
UAIUNKRWKOVEES-UHFFFAOYSA-N
Application
3,3′,5,5′-Tetramethylbenzidine has been used as a substrate for peroxidase in:
- enzyme-immunoassay (EIA)
- indirect competitive enzyme-linked immunosorbent assay (ELISA)
- sandwich enzyme-linked immunosorbent assay
3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.
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signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 4 - Carc. 2
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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