70014-M
T7Select® Packaging Kit
Ultimate efficiency in T7Select library construction
Synonym(s):
T7Select® Phage Display System, T7Select® System
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About This Item
UNSPSC Code:
41106617
NACRES:
NA.54
Quality Level
manufacturer/tradename
Novagen®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
shipped in
dry ice
storage temp.
−70°C
General description
Novagen′s T7Select® Phage Display System is a novel phage display system that takes advantage of the properties of bacteriophage T7. This system is easy to use and has the capacity to display peptides up to about 50 amino acids in size in high copy number (415 per phage), and peptides or proteins up to about 1200 amino acids in low copy number (0.1-1 per phage) or mid-copy number (5-15 per phage). Phage assembly takes place in the E. coli cytoplasm and mature phage are released by cell lysis. Unlike the filamentous systems, peptides or proteins displayed on the surface of T7 do not need to be capable of secretion through the cell membrane, a necessary step in filamentous phage assembly.
T7Select Packaging Extracts are optimized for maximum packaging efficiencies of T7Select vectors ligated with insert. The high efficiency extracts (> 2 x 108 pfu/µg with T7Select Packaging Control DNA) make it easy to produce a library containing at least 5 x 107 recombinants per microgram of arms. The extracts are made from a specially engineered phage that reduces the nonrecombinant cloning background to below 0.1%.
T7Select Packaging Extracts are optimized for maximum packaging efficiencies of T7Select vectors ligated with insert. The high efficiency extracts (> 2 x 108 pfu/µg with T7Select Packaging Control DNA) make it easy to produce a library containing at least 5 x 107 recombinants per microgram of arms. The extracts are made from a specially engineered phage that reduces the nonrecombinant cloning background to below 0.1%.
Components
- 6- T7 Packaging Extracts
- 1 µg- T7Select Packaging Control DNA
- 0.2 ml- BL21 glycerol stock
- 0.2 ml- BLT5403 glycerol stock
- 0.2 ml- BLT5615 glycerol stock
Warning
Toxicity: Standard Handling
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
T7SELECT is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Clickhere for Enduser Declaration (EUD) Form
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Certificates of Analysis (COA)
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