The installation process involves the following steps:
1.Install the guard cartridge into the holder as shown in the attached document.
2.Connect the tubing from the HPLC injector to the inlet of the guard column holder with the cone-shaped ferrule pointed down towards the end of the tubing.
3.Install the tubing/nut/ferrule provided in the kit to the outlet of the guard column holder, following the same process as described in step no. 2.
4.Connect the outlet tubing from the guard holder to the analytical HPLC column, again, following the same procedure as directed in step no. 2.
581373-U
Ascentis® C18 (5 µm) HPLC Columns
L × I.D. 2 cm × 4 mm, HPLC Guard Cartridge Kit with 1 Cartridge holder + 1 Guard Cartridge
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About This Item
UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
Pricing and availability is not currently available.
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Product Name
Ascentis® C18 Supelguard Guard Cartridge, Kit, 5 μm particle size, L × I.D. 2 cm × 4 mm
material
stainless steel column
Quality Level
Agency
suitable for USP L1
product line
Ascentis®
feature
endcapped
packaging
pkg of 1 kit
extent of labeling
25% Carbon loading
parameter
≤70 °C temp. range
technique(s)
HPLC: suitable
LC/MS: suitable
L × I.D.
2 cm × 4 mm
surface area
450 m2/g
impurities
5 ppm metals
matrix
fully porous particle
matrix active group
C18 (octadecyl) phase
particle size
5 μm
pore size
100 Å
operating pH range
2-8
application(s)
food and beverages
separation technique
reversed phase
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General description
Kit includes one cartridge, stand-alone holder, a piece of tubing, 2 nuts and 2 ferrules.
Footnote
Kit includes one cartridge, a stand-alone holder, a piece of tubing, 2 nuts and ferrules (10mm i.d guard requires the purchase of 567499-U)
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
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Protocols
Reversed-phase HPLC sometimes requires ion pairing for adequate retention of polar compounds due to hydrophobic interactions.
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