V3388
Vascular Endothelial Growth Factor (aa 207-318) human
≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder
Synonym(s):
VEGF112, Vasculotropin
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About This Item
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biological source
human
Quality Level
recombinant
expressed in E. coli
Assay
≥97% (SDS-PAGE)
form
lyophilized powder
potency
0.75-3.75 ng/mL ED50
mol wt
predicted mol wt ~28 kDa
packaging
pkg of 5 μg
storage condition
avoid repeated freeze/thaw cycles
impurities
<1 EU/μg
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... VEGFA(7422)
Application
Vascular Endothelial Growth Factor (aa 207-318) human also known as VEGF 121 was used for preparing a cellular matrix hybridized with hyaluronic acid (ACM-HA) VEGF constructs which is used in measuring the VEGF-enhanced tissue-engineered bladder graft neovascularization by using dynamic contrast-enhanced MRI. The product was used for creating VEGF-impregnated tissue-engineered construct which was inserted in the critical-sized calvarial defect in the membranous parietal bone of the rabbit for examining and characterizing the angiogenesis necessary for bone formation.
Biochem/physiol Actions
Vascular Endothelial Growth Factor (aa 207-318) human also referred to as VEGF 121 or Vasculotropin is a basic protein that induces endothelial cell growth, vasculogenesis and angiogenesis. VEGF also enhances the growth of endothelial cells isolated from cerebral cortex, fetal and adult aorta as well as human umbilical vein. Cells enhance VEGF 121 secretion from fibrin implants results in local and controlled blood vessel growth.
Potent angiogenesis inducer in vivo.
Other Notes
112 amino acid residue variant of human VEGF.
Physical form
Lyophilized from a 0.2 μm filtered solution in 30% acetronitrile and 0.1% TFA containing 0.25 mg bovine serum albumin.
Analysis Note
The biological activity is measured by its ability to stimulate 3H-thymidine incorporation in human umbilical vein endothelial cells.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Martin Ehrbar et al.
Circulation research, 94(8), 1124-1132 (2004-03-27)
Although vascular endothelial growth factor (VEGF) has been described as a potent angiogenic stimulus, its application in therapy remains difficult: blood vessels formed by exposure to VEGF tend to be malformed and leaky. In nature, the principal form of VEGF
Lisa Cartwright et al.
Journal of biomedical materials research. Part A, 77(2), 390-395 (2006-01-24)
Tissue engineered organs require an immediately perfused vascular tree. Currently, neovascularization assessment requires animal sacrifice and graft harvest. In this pilot study we assess whether neovascularization of an engineered urinary bladder construct is enhanced with vascular-derived endothelial growth factor (VEGF)
Leung, D.W., et al.
Science, 246 (1989)
Sevan Evren et al.
Cells, tissues, organs, 192(4), 250-261 (2010-07-01)
Natural scaffolds have been shown to induce T helper 2 (TH2)-specific immune responses in host tissues; however, the precise mechanisms that underlie this immune response are unknown. Using a porcine animal model, we evaluated the role of Toll-like receptors (TLRs)
Marine Beaumont et al.
NMR in biomedicine, 23(1), 48-55 (2009-08-04)
Tissue engineering is a promising technique for bone repair and can overcome the major drawbacks of conventional autogenous bone grafting. In this in vivo longitudinal study, we proposed a new tissue-engineering paradigm: inserting a biological soft-tissue construct within the bone
Articles
VEGF-A stimulates blood vessel growth via endothelial cell proliferation, migration, and survival during embryonic development.
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