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biological source
human
Assay
≥75% (SDS-PAGE)
form
frozen liquid
packaging
pkg of 2 μg
storage condition
avoid repeated freeze/thaw cycles
concentration
200 μg/mL
color
clear colorless
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... ERCC2(2068)
Biochem/physiol Actions
TFIIH is a multicomponent basal transcription factor complex. Nine subunits have been identified within the TFIIH holoenzyme complex. Various enzymatic activities, including DNA repair, helicase, and cyclin-dependent kinase activities, have been reported. The XPB, p62, p52, p44, and p34 subunits are thought to constitute the "core" of the TFIIH transcription machinery. Although the p44 and p34 subunits have no defined enzymatic activity, their zinc finger structures suggest that they may be DNA-binding proteins that might mediate interactions with soluble transcription factors. The cdk-activating kinase (CAK) subcomplex, comprising subunits Cdk7, cyclin H, amd MAT1, phosphorylate several cyclin-dependent kinases (cdks), as well as the carboxy-terminal domain of pol II. Several inherited human disorders such as Xeroderma pigmentosum (XP), Cockayne syndrome (CS) and trichothiodystrophy (TTD) are associated with mutations in TFIIH subunits.
Physical form
Clear and colorless frozen liquid solution
Preparation Note
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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O Flores et al.
The Journal of biological chemistry, 267(4), 2786-2793 (1992-02-05)
Two new factors required for transcription of class II genes have been identified. These factors, TFIIH and TFIIJ, were required together with the previously described general factors (TFIIA, TFIIB, TFIID, TFIIE, and TFIIF) and RNA polymerase II for transcription of
W J Feaver et al.
The Journal of biological chemistry, 266(28), 19000-19005 (1991-10-05)
Heat treatment of yeast nuclear extracts abolished the capacity to initiate transcription at RNA polymerase II promoters. Activity was restored by the addition of both recombinant yeast TFIID and partially purified factor b, a yeast fraction shown previously to be
R Drapkin et al.
Nature, 368(6473), 769-772 (1994-04-21)
The RNA polymerase II general transcription factor TFIIH is composed of several polypeptides. The observation that the largest subunit of TFIIH is the excision-repair protein XPB/ERCC3 (ref. 1), a helicase implicated in the human DNA-repair disorders xeroderma pigmentosum (XP) and
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