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SHC016

Sigma-Aldrich

MISSION® pLKO.1-puro Non-Target shRNA Control Plasmid DNA

Targets no known genes from any species

Synonym(s):

MISSION® Control Vectors, negative control, negative shRNA control, non-target control, non-target shRNA, non-target shRNA control, shRNA control

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About This Item

MDL number:
MFCD07785395
UNSPSC Code:
41106609
NACRES:
NA.51

product line

MISSION®

concentration

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

shipped in

dry ice

storage temp.

−20°C

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General description

The MISSION pLKO.1-puro Non-Target shRNA Control Plasmid DNA is a lentivirus plasmid vector. The vector contains an shRNA insert that does not target any known genes from any species, making it useful as a negative control in experiments using the MISSION shRNA library clones. This allows one to examine the effect of transfection of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The Non-Target shRNA Control Plasmid DNA is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.

Application

MISSION® pLKO.1-puro non-target shRNA control plasmid DNA has been used as a control during transduction:
  • in tumor cells for multicolour imaging
  • in human adult low calcium temperature keratinocytes
  • in mouse embryonic fibroblasts, to study the biological functions of IP6K1 (inositol hexakisphosphate kinase)
  • to study the function of Zac1 (zinc finger protein regulating apoptosis and cell cycle arrest) expression in astroglial differentiation
To see more application data, protocols, vector maps visit sigma.com/shrna.

Legal Information

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

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SHC003

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MISSION® pLKO.1-puro TurboGFP™ shRNA Control Plasmid DNA, shRNA sequence targeting tGFP

SHC005

MISSION® pLKO.1-puro eGFP shRNA Control Plasmid DNA, shRNA sequence targeting eGFP

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MISSION® pLKO.1-puro Luciferase shRNA Control Plasmid DNA, shRNA sequence targeting luciferase

SHC012

MISSION® pLKO.1-puro-CMV-TagRFP™ Positive Control Plasmid DNA, Contains a gene encoding TagRFP

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MISSION® pLKO.1-puro-UbC-TurboGFP™ Positive Control Plasmid DNA, Contains a gene encoding TurboGFP, under the control of the UbC promoter

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Deletion of inositol hexakisphosphate kinase 1 (IP6K1) reduces cell migration and invasion, conferring protection from aerodigestive tract carcinoma in mice
Jadav RS, et al.
Cellular Signalling, 28(8), 1124-1136 (2016)
Brain tumour cells interconnect to a functional and resistant network
Osswald M, et al.
Nature, 528(7580), 93-93 (2015)
Sophie Weil et al.
Neuro-oncology, 19(10), 1316-1326 (2017-04-19)
Primary and adaptive resistance against chemo- and radiotherapy and local recurrence after surgery limit the benefits from these standard treatments in glioma patients. Recently we found that glioma cells can extend ultra-long membrane protrusions, "tumor microtubes" (TMs), for brain invasion
Zac1 regulates astroglial differentiation of neural stem cells through Socs3
Schmidt EU, et al.
Stem Cells, 31(8), 1621-1632 (2013)
Peroxiredoxin 2 nuclear levels are regulated by circadian clock synchronization in human keratinocytes
Avitabile D, et al.
The International Journal of Biochemistry & Cell Biology, 53(7580), 24-34 (2014)
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