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SAB4500088

Sigma-Aldrich

Anti-Tubulin β antibody produced in rabbit

affinity isolated antibody

Synonym(s):

TUBB3, TUBB4, Tubulin β-3 chain, Tubulin β-4, Tubulin β-III

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About This Item

MDL number:
MFCD01322746
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 50 kDa

species reactivity

human, mouse, rat

concentration

~1 mg/mL

technique(s)

ELISA: 1:5000
immunohistochemistry: 1:50-1:100
western blot: 1:500-1:1000

NCBI accession no.

Q13509

UniProt accession no.

Q13509

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TUBB3(10381)

General description

Tubulin β-III (TUBB3) is a constituent of microtubules. It is expressed in mature neurons. It is located on human chromosome 16q24.

Anti-Tubulin β Antibody detects endogenous levels of total Tubulin β protein.

Immunogen

The antiserum was produced against synthesized peptide derived from human Tubulin beta.

Immunogen Range: 401-450

Application

Anti-Tubulin β C-Terminal antibody has been used in immunoblot assays.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)

Biochem/physiol Actions

Upregulation of tubulin β-III (TUBB3) shows vigorous tumor characteristics and genetic instability in urinary bladder cancer. It participates in the separation of chromosome. Individuals with congenital fibrosis of the extraocular muscle type 3 (CFEOM3) show mutations in TUBB3. βIII-tubulin modulates glucose metabolism, thereby transforms glucose starvation stress signaling to develop the multiplication of cell and survival in non-small cell lung cancer cells.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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?III-tubulin overexpression is linked to aggressive tumor features and genetic instability in urinary bladder cancer
Hinsch A, et al.
Human Pathology (2017)
Yi-Qing Gong et al.
Oncotarget, 7(37), 60123-60132 (2016-08-16)
Excessive Ultra-violet (UV) radiation causes oxidative damages and apoptosis in retinal pigment epithelium (RPE) cells. Here we tested the potential activity of SC79, a novel small molecule activator of Akt, against the process. We showed that SC79 activated Akt in
Chuchu Gong et al.
Journal of cellular and molecular medicine, 27(15), 2165-2182 (2023-06-30)
Sulfur mustard (SM) is a blister-producing chemical warfare agent which could lead to a cascade of systemic damage, especially severe acute lung injury. Oxidative stress is considered to be vital processes for the SM toxicity mechanism. We previously proved the
?III-Tubulin alters glucose metabolism and stress response signaling to promote cell survival and proliferation in glucose-starved non-small cell lung cancer cells
Parker AL, et al.
Carcinogenesis (2016)
Mutations in Human Tubulin Proximal to the Kinesin-Binding Site Alter Dynamic Instability at Microtubule Plus- and Minus-Ends
Ti SC, et al.
Developmental Cell (2016)
View All Related Papers

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