SAB4200697
Anti-Tyrosine Hydroxylase antibody, Mouse monoclonal
clone TH-16, purified from hybridoma cell culture
Synonym(s):
TH, TYH
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.44
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
TH-16, monoclonal
form
buffered aqueous solution
species reactivity
rat, dog, rabbit, human, sheep, guinea pig, mouse, monkey, bovine
concentration
~1 mg/mL
technique(s)
immunoblotting: 1-2 μg/mL using whole extract of rat PC-12 cells
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... TH(7054)
Related Categories
General description
Monoclonal Anti-tyrosine hydroxylase (mouse IgG1 isotype) is derived from the hybridoma TH-16 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified rat tyrosine hydroxylase. This antibody recognizes an epitope located in the N-terminal region of human, monkey, bovine, sheep, rabbit, dog, guinea pig, mouse and rat tyrosine hydroxylase (TH).
Tyrosine hydroxylase (TH) is encoded by the gene mapped to human chromosome 11p15. TH is a tetramer of four identical subunits, which is characterized with a regulatory, catalytic, and tetramerization domains. The enzyme utilizes tyrosine, BH4 and O2 as co-substrates, and Fe2+ as a cofactor.
Tyrosine hydroxylase (TH) is encoded by the gene mapped to human chromosome 11p15. TH is a tetramer of four identical subunits, which is characterized with a regulatory, catalytic, and tetramerization domains. The enzyme utilizes tyrosine, BH4 and O2 as co-substrates, and Fe2+ as a cofactor.
Immunogen
purified rat Tyrosine Hydroxylase
Biochem/physiol Actions
Tyrosine hydroxylase (TH) catalyzes the first rate limiting step in the biosynthesis of catecholamine neurotransmitter,that is, the conversion of L-tyrosine to L-dopa. Inhibition of TH by L-phenylalanine, might play a crucial role in phenylketonuria and block the synthesis of norepinephrine. Activity of TH can be regulated by phosphorylation. Decreased expression of TH is associated with various neuro-psychiatric diseases such as schizophrenia and Parkinson′s disease (PD).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2–8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation. Working dilution samples should be discarded if not used within 12 hours.
Other Notes
In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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recommended
Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Phosphorylation of tyrosine hydroxylase in situ at serine 8, 19, 31, and 40.
Haycock JW
The Journal of Biological Chemistry, 265, 11682-11691 (1990)
Role of N-terminus of tyrosine hydroxylase in the biosynthesis of catecholamines.
Nakashima A, et al.
Journal of neural transmission (Vienna, Austria : 1996), 116, 1355-1362 (2009)
Chun Chen et al.
NPJ Parkinson's disease, 7(1), 39-39 (2021-05-14)
Here we report the application of a mass spectrometry-based technology, imaging mass cytometry, to perform in-depth proteomic profiling of mitochondrial complexes in single neurons, using metal-conjugated antibodies to label post-mortem human midbrain sections. Mitochondrial dysfunction, particularly deficiency in complex I
Chang Chen et al.
Metabolic brain disease, 34(1), 203-212 (2018-11-15)
Parkinson's disease (PD) is a common chronic neurodegenerative disease and greatly affects the quality of PD patients' life. Current symptomatic treatment of PD is limited. There are no effective treatment and drugs that could radically cure PD. Increasing experimental evidence
The V81M variant of tyrosine hydroxylase is associated with more severe freezing of gait in Parkinson's disease.
Tekin I, et al.
Parkinsonism & Related Disorders, 86-90 (2016)
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