SAB4200380

Anti-LAYN antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-LAYN antibody produced in rabbit, Anti-Layilin
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~43 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): indirect immunofluorescence: 2.5-5.0 μg/mL using human A549 cells.; western blot: 1-2 μg/mL using whole extracts of HEK-293T cells overexpressing human LAYN.
• UniProt accession no.: Q6UX15
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... LAYN(143903)

Description

General description

Layilin (LAYN) is a cell surface hyaluronan receptor, originally identified as a binding partner of talin located in membrane ruffles of spreading cells. LAYN is widely expressed in different cell lines and tissues. This gene is mapped to human chromosome 11q.

Immunogen

synthetic peptide corresponding to an internal region of human LAYN, conjugated to KLH. The antibody is affinity-purified using the immunizing peptide immobilized on agarose.

Application

Anti-LAYN antibody produced in rabbit has been used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

Layilin (LAYN) binds to hyaluronan, a ubiquitous extracellular matrix component involved in the invasion of a variety of tumour cells, through its extracellular domain and to cytoskeleton-membrane linker proteins including Merlin and Radixin, through its cytoplasmic domain, implicating LAYN in cell adhesion and motility. LAYN is overexpressed in the human lung carcinoma A549. LAYN down-regulation by RNA interference, inhibits invasion and lymphatic metastasis of A549 cells.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable