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SAB1412026

Sigma-Aldrich

ANTI-EPHB2 antibody produced in mouse

clone 1F10, ascites fluid

Synonym(s):

CAPB, DRT, EPHB2, EPHT3, ERK

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200 μL
RM 2,107.00

RM 2,107.00


Estimated to ship on14 April 2025



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200 μL
RM 2,107.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

RM 2,107.00


Estimated to ship on14 April 2025


biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

1F10, monoclonal

mol wt

antigen 36.63 kDa

species reactivity

human

technique(s)

indirect ELISA: suitable
western blot: 1:500-1:1000

isotype

IgMκ

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... EPHB2(2048)

General description

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members. (provided by RefSeq)

Immunogen

EPHB2 (NP_059145, 226 a.a. ~ 325 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Sequence
CIANAEEVDVPIKLYCNGDGEWLVPIGRCMCKAGFEAVENGTVCRGCPSGTFKANQGDEACTHCPINSRTTSEGATNCVCRNGYYRADLDPLDMPCTTIP

Physical form

Clear solution

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Questions

  1. What is the typical concentration of antibodies, and in which solution is the antibody stored?

    1 answer
    1. It is supplied in "ascites fluid" and there is not any other solution composition/solvent the product is supplied in. The reason it supplied at "ascites fluid" is that the lab cannot get the purified antibody from the ascites by Protein A purification. Its antibody concentration would be around 0.4 ~ 0.8 mg/mL. This concentration is not the absolute concentration after purification, but a reference only.

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