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R1158

Sigma-Aldrich

Ribonuclease Inhibitor

recombinant, expressed in E. coli, 20-40 units/μL

Synonym(s):

RNAse inhibitor

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2500 UNITS
RM 1,057.00
10000 UNITS
RM 2,606.00

RM 1,057.00

Estimated to ship on24 March 2025

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2500 UNITS
RM 1,057.00
10000 UNITS
RM 2,606.00

About This Item

MDL number:
MFCD00132187
UNSPSC Code:
12352200
NACRES:
NA.52

RM 1,057.00

Estimated to ship on24 March 2025

recombinant

expressed in E. coli

Quality Level

300

grade

for molecular biology

form

buffered aqueous solution

mol wt

~50 kDa

concentration

20-40 units/μL

shipped in

dry ice

storage temp.

−20°C

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General description

Ribonuclease inhibitor works to inhibit RNase activity by forming a tight, non-covalent 1:1 complex. It is derived from E. coli which expresses portions of the human placental ribonuclease inhibitor. It inhibits RNases A, B, and C. It will not inhibit RNases H, 1, T1, S1 Nuclease, SP6, T7 RNA Polymerase, T3 RNA Polymerase, AMV Reverse Transcriptase, M-MLV Reverse Transcriptase, or Taq Polymerase. The inhibitor can be removed by phenol extraction or by heating to 65°C for 10 minutes. The pH range for inhibition is pH 5.5–9 (highestinhibition at pH 7–8).

Application

Ribonuclease Inhibitor is suitable for use in useful for in vitro inhibition of ribonucleases, including procedures like:

  • cDNA synthesis from mRNA
  • reverse transcriptase polymerase chain reaction (RT-PCR)
  • in vitro transcription and translation
  • mRNA protection from degradation[1]
  • formaldehyde cross-linked lncRNA purification[2]
  • RNA-fluorescence in situ hybridization (FISH)[3]
Useful for in vitro inhibition of ribonucleases, including procedures like cDNA synthesis, RT-PCR, and in vitro transcription and translation.

Biochem/physiol Actions

Inhibits RNase by forming a tight, non-covalent 1:1 complex.

Components

Ribonuclease Inhibitor is provided as a solution in 20 mM HEPES-KOH( pH 7.6), 50 mM KCl, 8 mM DTT and 50% glycerol.

Unit Definition

One unit will reduce the activity of 5 ng of ribonuclease A by 50% in a cytidine 2′:3′-cyclic monophosphate system.

Physical form

Solution in 20 mM HEPES-KOH, pH 7.6, 50 mM KCl, 8 mM DTT and 50% glycerol.

Other Notes

  • Denaturing conditions (i.e., urea or temperatures ≥50 °C) should be avoided as they may cause a release of active ribonuclease from the complex.
  • This product is for R&D use only, not for drug, household, or other uses.

related product

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000294497
SLCR2968
SLCR2968
0000294497
SLCP1993

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Questions

  1. What is the difference between R1158 and RNAINH-RO?

    1 answer

    1. Both are similar products. However, Product R1158 is a Sigma brand product with an activity specification range of 20 - 40 units//uL, while product RNAINH-RO is a Roche manufactered product with an activity specification of >40 units/ul. See the links below to review the product datasheet for each item:
      R1158
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/139/860/r1158dat.pdf

      RNAINH-RO
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/647/411/rnainh-ro.pdf

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