P9489

Anti-p35 (Cdk5 Regulator) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): Anti-CDK5P35, Anti-CDK5R, Anti-NCK5A, Anti-p23, Anti-p25, Anti-p35, Anti-p35nck5a
• MDL number: MFCD00211612
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 35 kDa
• species reactivity: human, rat
• technique(s): microarray: suitable; western blot: 1:1,000 using a rat brain extract (cytosolic fraction)
• UniProt accession no.: Q15078
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CDK5R1(8851) ; rat ... Cdk5r1(116671)

Description

General description

Cell division protein kinase 5 (Cdk5) is a serine/threonine kinase and shares homology to other Cdks. It belongs to cdk family.

Immunogen

synthetic peptide corresponding to the C-terminus of p35 (Cdk5R), (human, amino acids 192-209).

Application

Anti-p35 (Cdk5 Regulator) antibody produced in rabbit has been used in western blotting and immunohistochemistry.

Biochem/physiol Actions

Cell division protein kinase 5 (Cdk5) kinase activity can be detected mainly in postmitotic neurons in the central nervous system (CNS). Cdk5/p35 activity is required in the mature CNS. Cdk5 phosphorylates dopamine and cyclic adenosine 3′,5′-monophosphate-regulated phosphoprotein of 32 kDa (DARPP-32) and Syntaxin-binding protein 1 (Munc18), which in turn affects synaptic vesicle endocytosis. Deregulation of Cdk5 activity contributes to the pathogenesis of neurodegenerative diseases such as Alzheimer′s disease (AD). It mediates neuronal migration during cortical development. Cdk5-/- mice exhibit embryonic lethality associated with disruption of the cortical laminar structures in the cerebral cortex, olfactory bulb, hippocampus, and cerebellar cortex. Mice lacking p35 display defects in cortical lamination and fasciculation of axonal fibers. In developing mice brain, Cdk5/p35 interacts with Reelin/Dab1 and positions of cortical neurons.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.