This product is not tested for the presence of contaminating enzymes. The primary components are endo-polygalacturonase and endo-pectin lyase. A brief search of the literature shows that in protoplast isolation, cellulase is often added to the digest, in addition to pectolyase.
P3026
Pectolyase from Aspergillus japonicus
lyophilized powder, ≥0.3 units/mg solid
Synonym(s):
Pectolytic enzyme
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100 MG
RM 1,135.00
250 MG
RM 2,093.00
1 G
RM 5,647.00
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100 MG
RM 1,135.00
250 MG
RM 2,093.00
1 G
RM 5,647.00
About This Item
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Recommended Products
biological source
Aspergillus sp. (A. japonicus)
Quality Level
form
lyophilized powder
specific activity
≥0.3 units/mg solid
mol wt
50 kDa
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storage temp.
2-8°C
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General description
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research.
Pectolyase or pectin lyase is stable in the temperature range of 40-50°C and is a 50 kDa enzyme with an isoelectric pH of 3.8.[1]
Pectolyase or pectin lyase is stable in the temperature range of 40-50°C and is a 50 kDa enzyme with an isoelectric pH of 3.8.[1]
Application
Pectolyase from Aspergillus japonicus has been used:
- in the lysis of chlorella cells for protoplast generation[2]
- as component of enzyme digestion buffer for Arabidopsis meiotic chromosome isolation[3]
- to induce reorganization of Arabidopsis root cap cell architecture[4]
Used in plant protoplast preparation to digest cell wall prior to organelle isolation. It is used in complex carbohydrate analysis. Product P3026 is provided in a lyophilized powder containing lactose. Product P3026 has been used to digest Crassula root tips prior to meiotic analysis[5].
Used in plant protoplast preparation to digest cell wall prior to organelle isolation.
Biochem/physiol Actions
Pectolyase catalyzes the eliminative cleavage of a-(1- 4)-Dgalacturonan methyl ester to give oligosaccharides with 4-deoxy-6-O-methyl-a-D-galact-4-enuronosyl groups at their non-reducing ends. It contain two types of pectinase, endopolygalacturonase, endo-pectin lyase and a maceration stimulating factora.
Other Notes
Reported to contain two types of pectinase, endopolygalacturonase (EC3.2.1.15), endopectin lyase (EC4.2.2.10) and a maceration stimulating factor.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Unit Definition
One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 5.5 at 25 °C.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Protocol for the preparation of Arabidopsis meiotic chromosome spreads and fluorescent in situ hybridization
Bolanos-Villegas P, et al.
The Plant Journal, 4, e1102-e1102 (2014)
Pectic enzymes in pectolyase: separation, characterization, and induction of ethylene in fruits
Baldwin EA and Pressey R
Plant Physiology, 90(1), 191-196 (1989)
Isolation and properties of pectinases from the fungus Aspergillus japonicus
Semenova MV, et al.
Biochemistry (Moscow), 68(5), 559-569 (2003)
Identification of sporopollenin as the outer layer of cell wall in microalga Chlorella protothecoides
He X, et al.
Frontiers in Microbiology, 7, 1047-1047 (2016)
Susann Hesse et al.
Frontiers in plant science, 10, 773-773 (2019-07-10)
During prophase I a meiosis-specific proteinaceous tripartite structure, the synaptonemal complex (SC), forms a scaffold to connect homologous chromosomes along their lengths. This process, called synapsis, is required in most organisms to promote recombination between homologs facilitating genetic variability and
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Hello! I want to ask how much cellulase does the pectolyase (P3026) contains? It does not mention any contamination of other enzymes in the product description, but I want to know if there is any potential contamination involved.
1 answer-
Helpful?
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Hello, I wanted to ask if this enzyme is equivalent to pectolyase Y-23, as the activity values are expressed differently.
1 answer-
Yes, this product is equivalent to pectolyase Y-23.
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