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N4888

Sigma-Aldrich

Nutrient Mixture F-12 Ham

With sodium bicarbonate, without ʟ-glutamine, sterile liquid, suitable for cell culture

Synonym(s):

Ham’s F-12

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About This Item

MDL number:
MFCD00217417
UNSPSC Code:
12352207
NACRES:
NA.75

product name

Nutrient Mixture F-12 Ham, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: yes
sodium pyruvate: 0.11 g/L
phenol red: yes
L-glutamine: no
HEPES: no

shipped in

ambient

storage temp.

2-8°C

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General description

Nutrient Mixture F-12 is suitable for the growth of primary rat hepatocytes and rat prostate epithelial cells. It is also used in a clonal toxicity assay using Chinese hamster ovary (CHO) cells.

Application

Nutrient Mixture F-12 Ham has been used:
  • in Minimum Essential Medium to maintain SH-SY5Y human neuroblastoma cells
  • to culture female-originated neuroblastoma cells
  • to culture primary retinal pigment epithelium (RPE) cells and RPE cell lines

Reconstitution

Supplement with 0.146 g/L L-glutamine.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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