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MAK535

Sigma-Aldrich

Glutathione Reductase Kit

sufficient for 100 colorimetric tests

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

Quality Level

detection method

colorimetric

relevant disease(s)

neurological disorders; cardiovascular diseases; hematological disorder; cancer

shipped in

wet ice

storage temp.

−20°C

Related Categories

General description

Glutathione reductase (GR) reduces oxidized glutathione (GSSG) to the reduced sulfhydryl form GSH which is an important cellular antioxidant. A high GSH/GSSG ratio is important for protection against oxidative stress. Thus, measurement of GR activity is used as indicator for oxidative stress

Features and Benefits

  • Linear detection range: 0.4 to 50 U/L
  • Compatible with high-throughput handling systems.

Suitability

The kit is suitable for glutathione reductase activity determination in biological samples (e.g. plasma, serum, tissue, and culture media).

Principle

The Glutathione Reductase Kit is designed to accurately measure GR activity in biological samples with a protocol that utilizes Ellman′s method, in which DTNB reacts with the GSH generated from the reduction of GSSG by the GR in a sample to form a yellow product (TNB2-). The rate of change in the optical density, measured at 412 nm, is directly proportional to GR activity in the sample.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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