MAK361
Phosphatidylethanolamine Assay Kit (Fluorometric)
sufficient for 100 fluorometric tests
Synonym(s):
Fluorometric Assay Kit for Phosphatidylethanolamine
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About This Item
UNSPSC Code:
12352200
Recommended Products
usage
sufficient for 100 fluorometric tests
detection method
fluorometric
relevant disease(s)
cancer; aging/geriatric diseases
storage temp.
−70°C
General description
Phosphatidylethanolamine (PE), also known as cephalin is the second most abundant phospholipid in animal and plant tissues and is present on the cytoplasmic side of the plasma membrane. Phosphatidylethanolamine is a neutral phospholipid consisting of a phosphatidyl group ester linked to an ethanolamine molecule. Its functions include membrane fission/fusion, maintenance of membrane curvature and stabilization of membrane proteins, since it can form hydrogen bonds with proteins through an ionizable amine group. It acts as a chaperone during assembly of membrane proteins and aids in their translocation from the cytoplasm to the membrane. It is also involved in secretion of very low-density lipoproteins in the liver.
Suitability
Suitable for the measurement of Phosphatidylethanolamine content in lipid extracts from cells and tissues.
Principle
The Phosphatidylethanolamine assay kit is a microplate based enzymatic assay for the quantitation of PE in cells and tissues. PE Converter hydrolyses PE to an intermediate, which converts a colorless probe to a fluorescent product via enzymatic reaction (λex = 535 nm/λem = 587 nm). The intermediate formed through PE converter hydrolysis is specific to phosphatidylethanolamine. No other phospholipids (i.e. phosphatidylcholine, phosphatidylinositol or phosphatidic acid) will be detected, making the kit highly specific. The assay kit can detect as low as 0.2 nmol per well.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
10 - Combustible liquids
Flash Point(F)
188.6 °F
Flash Point(C)
87 °C
Certificates of Analysis (COA)
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