Yes, gelation is pH dependent. Adjusting the pH to 5.7 is recommended prior to heating to melt the agar and autoclaving to sterilize. Please see the link below to review the general plant culture medium preparation instructions available in the datasheet for product M5519 (formulation without agar):
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/361/537/m5519pis.pdf
M9274
Murashige and Skoog Basal Medium
suitable for plant cell culture, with sucrose and agar
Synonym(s):
MS Basal Medium
Sign Into View Organizational & Contract Pricing
All Photos(1)
Select a Size
Change View
1 L
RM 84.00
10 L
RM 297.00
About This Item
Recommended Products
Quality Level
form
powder
technique(s)
cell culture | plant: suitable
application(s)
agriculture
shipped in
ambient
storage temp.
2-8°C
Looking for similar products? Visit Product Comparison Guide
Application
Formula variant
With the macro- and micronutrients, vitamins, sucrose and agar as described by Murashige and Skoog (1962).
Media Formulation
Media Formulation
Quantity
Formulated to contain 42.4 grams of powder per liter of medium.
related product
Product No.
Description
Pricing
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Ox. Sol. 3
Storage Class Code
5.1B - Oxidizing hazardous materials
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Baba Ngom et al.
Plant signaling & behavior, 12(9), e1356967-e1356967 (2017-08-02)
Cytosine DNA methylation is an epigenetic regulatory system used by plants to control gene expression. Methylation pattern always changes after abiotic stresses, pathogens and pest infections or after a treatment with salicylic acid (SA). The latter is a key player
Antoine Fort et al.
The New phytologist, 209(2), 590-599 (2015-09-24)
Heterosis is the phenomenon whereby hybrid offspring of genetically divergent parents display superior characteristics compared with their parents. Although hybridity and polyploidy can influence heterosis in hybrid plants, the differential contributions of hybridity vs polyploidy to heterosis effects remain unknown.
Striking effects of melatonin on secondary metabolites produced by callus culture of rosemary (Rosmarinus officinalis L.)
Coskun Y, et al.
Plant Cell, Tissue and Organ Culture, 138(1), 89-95 (2019)
Laure Audonnet et al.
Gene expression patterns : GEP, 25-26, 1-7 (2017-04-13)
Dimethylation of histone H3 lysine 9 (H3K9me2) is a heterochromatic mark linked to DNA methylation and gene repression. Removal of H3K9me2 from gene bodies by the jmjC histone demethylase IBM1/JMJ25 inhibits DNA methylation and derepresses gene expression. In this work
P Panichayupakaranant et al.
Pharmaceutical biology, 48(11), 1192-1197 (2010-09-17)
Rhinacanthus nasutus (L.) Kurz (Acanthaceae) has long been used in Thai traditional medicine for treatment of tinea versicolor, ringworm, pruritic rash, and abscess. The active constituents are known as a group of naphthoquinone esters, rhinacanthins. This work focused on establishment
-
Would an incorrect pH level affect the setting of the agar?
1 answer-
Helpful?
-
-
How firm is the gel in the final sterile medium preparation of Murashige and Skoog Basal Medium, Product M9274?
1 answer-
This formulation contains 8g/L of standard agar resulting in fairly firm and rigid culture medium.
Helpful?
-
-
What is the stability of the prepared Murashige and Skoog Basal Medium, Product M9274?
1 answer-
This agar-containing medium can be stored for 3 to 6 months, depending on the stability of any additional supplements, if stored properly with no observable dehydration of the gel. If the medium appears to darken or pull away from the sides of the culture vessel, it should be discarded.
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
How is Murashige and Skoog Basal Medium, Product M9274 different from Product M5519?
1 answer-
M9274 contains sucrose at 30 g/L and agar at 8 g/L; M5519 does not.
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
