L1378
L-Lactic Dehydrogenase from bovine muscle
Type X, ammonium sulfate suspension, ≥600 units/mg protein
Synonym(s):
(S)-Lactate: NAD+ oxidoreductase, L-LDH, LAD, LD, Lactate
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
Recommended Products
biological source
bovine muscle
type
Type X
Assay
3.0—25.0 mg protein/mL (biuret)
form
ammonium sulfate suspension
specific activity
≥600 units/mg protein
manufacturer/tradename
Sigma-Aldrich
technique(s)
activity assay: suitable
color
white
pH
7.5
application(s)
life science and biopharma
foreign activity
pyruvate kinase ≤0.01%
storage temp.
2-8°C
Gene Information
cow ... LDHA(281274) , LDHB(281275)
General description
Research Area: Cell Signaling
Lactate dehydrogenase (LDH) is a key enzyme in the anaerobic metabolic pathway and falls under the class of oxidoreductases. This enzyme is found in a wide range of organisms, including both plants and animals. It is widely distributed across all tissues. LDH is primarily a cytoplasmic enzyme, with particularly high concentrations in muscle, liver, and kidney tissues.
Lactate dehydrogenase (LDH) is a key enzyme in the anaerobic metabolic pathway and falls under the class of oxidoreductases. This enzyme is found in a wide range of organisms, including both plants and animals. It is widely distributed across all tissues. LDH is primarily a cytoplasmic enzyme, with particularly high concentrations in muscle, liver, and kidney tissues.
Application
L-Lactic Dehydrogenase from bovine muscle has been used in lactate assay to assess lactate tissue accumulation.
Biochem/physiol Actions
Lactate dehydrogenase (LDH) plays a critical role as a checkpoint in gluconeogenesis and DNA metabolism. It plays a vital role in maintaining homeostasis during periods of low oxygen availability. As an oxidoreductase enzyme, lactate dehydrogenase catalyzes the reversible conversion of pyruvate to lactate using NADH. This enzyme is crucial for the anaerobic metabolism of glucose when oxygen is either absent or in short supply. LDH is the sole serum biomarker effective for evaluating metastatic melanomas.
Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.
Unit Definition
One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.
Physical form
Crystalline suspension in 2.4 M (NH4)2SO4 solution, pH 6.0
Analysis Note
Protein determined by biuret.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Biochemistry, lactate dehydrogenase
Farhana A and Lappin SL
StatPearls [Internet] (2020)
Metabolic recovery from submaximal exercise in hypoxia acclimated high altitude deer mice (Peromyscus maniculatus)
Dessureault LM, et al.
Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, 274, 111004-111004 (2024)
Deshdeepak Sahni et al.
Journal of neurosurgery. Pediatrics, 11(5), 575-583 (2013-03-12)
Graphene possesses unique electrical, physical, and chemical properties that may offer significant potential as a bioscaffold for neuronal regeneration after spinal cord injury. The purpose of this investigation was to establish the in vitro biocompatibility of pristine graphene for interface
Wei Quan et al.
Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan, 33(1), 85-91 (2013-04-20)
To determine the cardioprotective effect of magnesium lithospermate B (MLB) on myocardial ischemia/reperfusion (MI/R) injury and to investigate the antioxidant potential in vivo and in vitro. MI/R injury was induced by the occlusion of left anterior descending coronary artery for
Vasco Meneghini et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 33(14), 6047-6059 (2013-04-05)
Dysregulated hippocampal neurogenesis has been associated with neurodegenerative disorders, including Alzheimer's disease (AD), in which it may potentially represent an auto-reparatory mechanism that could counteract neuronal loss and cognitive impairment. We evaluated hippocampal neurogenesis in TgCRND8 mice and reported that
Articles
Instructions for working with enzymes supplied as ammonium sulfate suspensions
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service