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FLAAM

Sigma-Aldrich

Adenosine 5′-triphosphate (ATP) assay mix

lyophilized powder

Synonym(s):

ATP assay kit, ATP detection kit

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1 VIAL
RM 2,728.00
5 VIALS
RM 9,069.00
10 VIALS
RM 16,623.00

RM 2,728.00


Estimated to ship on30 May 2025



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1 VIAL
RM 2,728.00
5 VIALS
RM 9,069.00
10 VIALS
RM 16,623.00

About This Item

UNSPSC Code:
12352202
eCl@ss:
32160414
NACRES:
NA.84

RM 2,728.00


Estimated to ship on30 May 2025


form

lyophilized powder

usage

1 vial sufficient for ≥50 assays

technique(s)

PCR: suitable

storage temp.

−20°C

General description

The Adenosine 5’-triphosphate (ATP) Bioluminescent Assay Kit may be employed for the quantitative bioluminescent determination of adenosine 5’-triphosphate (ATP) in samples containing 2x10–12 to 8x10–5 moles/liter. ATP is consumed and light is emitted when luciferase catalyzes the oxidation of D-luciferin.

Application

Adenosine 5′-triphosphate (ATP) assay mix has also been used to measure the level of cellular ATP in ATP viability assay.[1][2][3]
The ATP assay kit has been used to measure the level of cellular ATP present in isolated mitochondrial fractions from L. donovani promastigotes. [4]

Components

Contains luciferase, luciferin, MgSO4, EDTA, DTT, BSA, and Tricine buffer.

Reconstitution

Reconstitute with 5 ml of sterile water.

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Eye Dam. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Nobuyuki Nishikawa et al.
Neurourology and urodynamics, 39(6), 1644-1652 (2020-06-13)
Bladder wall stretch increases tissue tension and releases adenosine 5'-triphosphate (ATP) as part of a transduction process to sense bladder filling. Aging is associated with bladder fibrosis to produce a stiffer bladder wall: this may augment ATP release and contribute
Nazlıhan Aztopal et al.
Chemico-biological interactions, 280, 51-58 (2017-12-12)
Cancer stem-like cells (CSCs) are a cell subpopulation that can reinitiate tumors, resist chemotherapy, give rise to metastases and lead to disease relapse because of an acquired resistance to apoptosis. Especially, epigenetic alterations play a crucial role in the regulation
Ann K Rosenthal et al.
Arthritis research & therapy, 15(5), R154-R154 (2013-11-30)
Extracellular ATP (eATP) is released by articular chondrocytes under physiological and pathological conditions. High eATP levels cause pathologic calcification, damage cartilage, and mediate pain. We recently showed that stable over-expression of the progressive ankylosis gene product, ANK, increased chondrocyte eATP
Amit Roy et al.
Molecular pharmacology, 74(5), 1292-1307 (2008-08-16)
Mitochondria are the principal site for the generation of cellular ATP by oxidative phosphorylation. F0F1-ATP synthase, a complex V of the electron transport chain, is an important constituent of mitochondria-dependent signaling pathways involved in apoptosis. In the present study, we
Asako Kusumi et al.
Frontiers in microbiology, 2, 104-104 (2011-07-13)
To characterize sulfate-producing microorganisms from the deteriorated sandstones of Angkor monuments in Cambodia, strains of Mycobacterium spp. were isolated from most probable number-positive cultures. All five strains isolated were able to use both elemental sulfur (S(0)) for chemolithoautotrophic growth and

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