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F1879

Sigma-Aldrich

Formaldehyde Dehydrogenase from Pseudomonas sp.

lyophilized powder, 1.0-6.0 units/mg solid

Synonym(s):

FDH, Formaldehyde:NAD+ oxidoreductase

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About This Item

CAS Number:
Enzyme Commission number:
MDL number:
UNSPSC Code:
12352204

form

lyophilized powder

specific activity

1.0-6.0 units/mg solid

storage temp.

−20°C

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General description

Formaldehyde Dehydrogenase (FDH) belongs to the medium-chain alcohol dehydrogenase family.[1] It is encoded by the fdhA and fdhB gene in Pseudomonas aeruginosa.[2] The FDH sequences of P. aeruginosa and P. putida are conserved and show homology.[1] FDH exists as dimer and tetramer with zinc in their catalytic active site. The monomer corresponds to 350-400 amino-acid residues.[3]

Application

Formaldehyde Dehydrogenase from Pseudomonas sp. has been used:

  • For standard curve generation for the demethylation-FDH assay of lysine specific demethylase 1 (LSD1)[4] and in formaldehyde dehydrogenase (FDH) assay.[5]
  • As a biosensor for the presence of formaldehyde in pharmaceuticals, waste water, vaccines and industrial products.[6]
  • In coupled pectin methyl esterase (PME) enzyme assay.[6]

Biochem/physiol Actions

Formaldehyde dehydrogenase (FDH) is an enzyme that catalyzes the oxidation of formaldehyde to formate with NAD+ as an electron acceptor.[1]Formaldehyde dehydrogenases are involved in the biological detoxification of formaldehyde, a toxic product in the metabolism of compounds like histidine, vanillate etc. They detoxify by converting formaldehyde to carbon-di-oxide.[2] FDH plays a crucial role in regulating protein S-nitrosation in mammalian cells by decomposing S-nitrosoglutathione.[7] They are also involved in the regulation of nitrosothiol homeostasis by catalyzing the reduction of endogenous nitrosylating agent S-nitrosoglutathione (GSNO).[8]

Unit Definition

One unit will oxidize 1.0 μmole of formaldehyde to formic acid per min at pH 7.5 at 37 °C.

Physical form

Lyophilized powder containing ~70% stabilizers as Mg2+, Ca2+, bovine serum albumin, glycine, and lysine

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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In Vitro Enzyme Assays for JmjC-Domain-Containing Lysine Histone Demethylases (JmjC-KDMs)
Tarhonskaya H, et al.
Current protocols in pharmacology, 80(1), 3-15 (2018)
Structure of formaldehyde dehydrogenase from Pseudomonas aeruginosa: the binary complex with the cofactor NAD+
Liao Y, et al.
Acta Crystallographica Section F, Structural Biology and Crystallization Communications, 69(Pt 9), 967-972 (2013)
Slobodanka Grsic-Rausch et al.
Analytical biochemistry, 333(1), 14-18 (2004-09-08)
Pectin methylesterase (PME; EC 3.1.1.11) activities are widespread in bacteria, fungi, and plants. PME-mediated changes in cell wall pectin structure play important roles in plant development. Genome sequencing projects have revealed the existence of large PME multigene families in higher
Expression, purification, and characterization of formaldehyde dehydrogenase from Pseudomonas aeruginosa
Zhang W, et al.
Protein Expression and Purification, 92(2), 208-213 (2013)
Ivan V Rosado et al.
Nature structural & molecular biology, 18(12), 1432-1434 (2011-11-15)
Metabolism is predicted to generate formaldehyde, a toxic, simple, reactive aldehyde that can damage DNA. Here we report a synthetic lethal interaction in avian cells between ADH5, encoding the main formaldehyde-detoxifying enzyme, and the Fanconi anemia (FA) DNA-repair pathway. These

Questions

  1. この酵素の活性値を測定した方法について教えてください。

    1 answer
    1. The activity was found by taking the units/ml of enzyme and dividing it by the mg protein/ml of enzyme. The activity was found in an spectrophometric rate determination assay. The result found that one unit of enzyme will oxidize 1.0 micro mole of formaldehyde to formic acid per minute at pH 7.5 at 37°C.

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