E4788
Plant RNA/DNA Purification Kit
sufficient for 50 purifications
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About This Item
UNSPSC Code:
41105501
NACRES:
NA.55
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usage
sufficient for 50 purifications
Quality Level
greener alternative product characteristics
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General description
Norgen Biotek′s Plant RNA/DNA Purification Kit provides a rapid method for the isolation and purification of total RNA and genomic DNA simultaneously from a single sample of plants. The total RNA and genomic DNA are both column purified in under 30 minutes using a single column. It is often necessary to isolate total RNA and genomic DNA from a single plant sample, such as for studies of gene expression, mutant or transgenic plant characterization, and host plant-pathogen characterization. Traditionally the RNA and DNA would be isolated from different aliquots of a sample, however this novel technology will allow for their simultaneous isolation from the same sample. This will not only save time, but will also be of a great benefit when isolating RNA and DNA from precious, difficult to obtain or very small samples. Furthermore, gene expression analysis will be more reliable since the RNA and DNA are derived from the same sample, therefore eliminating inconsistent results.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
Application
For simultaneous isolation of total RNA and DNA from the same plant sample.
Purified RNA and DNA are of the highest quality and can be used in a number of downstream applications including:
- Real-time PCR
- Southern blotting
- SNP analysis
- Sequencing
- AFLP
- RFLP
- RAPD
- Microsatellite analysis
- qRT-PCR
- Primer extension
- Expression array assays
- Next Generation Sequencing
Features and Benefits
- Total RNA and genomic DNA are column purified simultaneously in under 30 minutes.
- Reduces variability as RNA and DNA are isolated from a single plant sample.
- Isolate nucleic acids from small plant samples.
- Isolates total RNA including microRNAs.
- No phenol/chloroform extraction or alcohol precipitation.
- Isolate DNA-free plant RNA or RNA-free plant DNA
Other Notes
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2
Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
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Ira Marton et al.
Plant physiology, 154(3), 1079-1087 (2010-09-30)
Zinc finger nucleases (ZFNs) are a powerful tool for genome editing in eukaryotic cells. ZFNs have been used for targeted mutagenesis in model and crop species. In animal and human cells, transient ZFN expression is often achieved by direct gene
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