For this product, the enzyme dilution factor is the factor by which the enzyme is diluted before the assay. The NAG Control Enzyme bulletin specifies a 1:100 dilution in Dilution Buffer, giving a dilution factor of 100. If the sample is undiluted, use 1; if diluted differently, use that actual dilution factor. Please see the link below to access the product datasheet, page 3, to review the recommended Activity Calculations:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/236/582/cs0780pis-ms.pdf
CS0780
β-N-Acetylglucosaminidase Assay Kit
sufficient for 50 reactions (1 mL), sufficient for 500 reactions (100 μL)
Synonym(s):
Beta-NAG Activity Assay Kit
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About This Item
UNSPSC Code:
12161503
EC Number:
222-398-7
NACRES:
NA.84
Pricing and availability is not currently available.
Quality Segment
usage
sufficient for 50 reactions (1 mL), sufficient for 500 reactions (100 μL)
shipped in
wet ice
storage temp.
2-8°C
Gene Information
human ... NAGLU(4669)
Application
The kit provides the reagents required for a fast and convenient detection of β-N-Acetylglucosaminidase activity in cell lysates, tissue homogenates or purified enzyme. It is a useful tool for the detection of lysosomes in fractionated cell/tissue samples.
Biochem/physiol Actions
N-acetyl-D-glucosaminidase (NAG) in mammals is a lysosomal enzyme, which takes part in the intracellular degradation of glycolipids and glycoproteins. High activities of this enzyme have been detected in human kidney, lung and liver lysosomes. Elevated levels of serum NAG are associated with certain forms of cancer.
Analysis Note
The kit assay is based on the hydrolysis of NAG substrate (NP-GLcNAc) by the enzyme. The enzymatic hydrolysis of the substrate liberates p-nitrophenylate ion. The reaction product is detected colorimetrically at 405 nm.
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Kit Components Only
Product No.
Description
- Dilution Buffer 8 mL
- 4-Nitrophenyl-N-acetyl-β-D-glucosaminide 50 mg
- Citrate Buffer Solution, 0.09 M 100 mL
- p-Nitrophenol Standard Solution, 10 mM 1 mL
- β-N-Acetylglucosaminidase from Jack beans 1 vial
- Sodium carbonate 5 g
Signal Word
Warning
Hazard Codes
Precautionary Statements
Hazard Classifications
Carc. 2 - Eye Irrit. 2 - STOT RE 2 Oral
Target Organs
Liver,Kidney
WGK
WGK 3
Storage Class
10 - Combustible liquids
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What's the enzyme dilution factor in the formula ?
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What are the lower and upper limits of enzyme activity that the CS0780 B-N-Acetylglucosaminidase Assay Kit can measure?
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The kit does not have specified lower and upper detection limits for enzyme activity. The enzyme activity needs to be empirically determined using varying enzyme amounts.
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