CS0030
Senescence Cells Histochemical Staining Kit
sufficient for 100 tests
Synonym(s):
Senescent cells IHC kit, cellular β-galactosidase probe
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.32
Recommended Products
usage
sufficient for 100 tests
Quality Level
packaging
pkg of 1 kit
storage condition
dry at room temperature
detection method
colorimetric
shipped in
dry ice
storage temp.
−20°C
Related Categories
General description
The Senescence Cells Histochemical Staining Kit contains all the reagents required for identifying senescent cells using an assay based on a histochemical stain for ß-galactosidase activity at pH 6. ß-galactosidase activity is detectable in senescent cells, but not in quiescent, immortal, or tumor cells.
The Senescence Cells Histochemical Staining Kit contains all the reagents required for identifying senescent cells using an assay based on a histochemical stain for β-galactosidase activity at pH 6. β-galactosidase activity is detectable in senescent cells, but not in quiescent, immortal, or tumor cells. Cellular senescence is a progression of events, whereby cells move from an actively dividing to a non-dividing stage. The cellular senescence process is associated with aging. The decrease in cell division is virtually irreversible and complete. In conjunction with the loss of the ability to divide, changes occur in the morphology, shape, and physical appearance of the cells, and their pattern of gene expression. At the end of the process cell death usually occurs, although the cells may remain viable for a long time.
Application
Senescence Cells Histochemical Staining Kit has been used for:
- senescence-associated β-galactosidase assay
- to test for senescence in human pancreatic cancer cells
- adipose-derived stem cells (ADSCs)
- cord blood mesenchymal stromal/stem cells (CBMSC)
Biochem/physiol Actions
Replicative senescence is a growth-arrest state associated with loss of division potential, changes in cell morphology, shape and physical appearance, and the pattern of gene expression in cells.
Legal Information
Manufactured under license to US Patent Nos. 5,491,069 and 5,795,728.
Kit Components Only
Product No.
Description
- X-gal solution 4 mL
- Staining Solution, 10X 15 mL
- Fixation Buffer 10x 15 mL
- Reagent B 1.5 mL
- Reagent C 1.5 mL
- Dulbecco's Phosphate Buffered Saline (PBS) 10x 60 mL
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Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Inhalation - Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Muta. 2 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
Target Organs
Respiratory system
Supplementary Hazards
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Certificates of Analysis (COA)
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B van der Loo et al.
Experimental cell research, 241(2), 309-315 (1998-06-25)
A beta-galactosidase activity has recently been used as a histochemical marker of replicative senescence in human fibroblasts and keratinocytes. To establish whether this marker could be used to detect senescence of vascular cells, we have investigated its presence in cultures
G P Dimri et al.
Proceedings of the National Academy of Sciences of the United States of America, 92(20), 9363-9367 (1995-09-26)
Normal somatic cells invariably enter a state of irreversibly arrested growth and altered function after a finite number of divisions. This process, termed replicative senescence, is thought to be a tumor-suppressive mechanism and an underlying cause of aging. There is
Mandy Oj Grootaert et al.
Autophagy, 11(11), 2014-2032 (2015-09-24)
Autophagy is triggered in vascular smooth muscle cells (VSMCs) of diseased arterial vessels. However, the role of VSMC autophagy in cardiovascular disease is poorly understood. Therefore, we investigated the effect of defective autophagy on VSMC survival and phenotype and its
Natalja E Fedorovich et al.
Biomaterials, 30(3), 344-353 (2008-10-22)
Photopolymerizable hydrogels, formed by UV-exposure of photosensitive polymers in the presence of photoinitiators, are widely used materials in tissue engineering research employed for cellular entrapment and patterning. During photopolymerization, the entrapped cells are directly exposed to polymer and photoinitiator molecules.
Phuc H Pham et al.
In vitro cellular & developmental biology. Animal, 53(1), 20-32 (2016-09-03)
As diseases and abnormalities of the heart can interfere with the aquaculture of Atlantic salmon, the heart was investigated as a source of cell lines that could be used to study the cellular basis of these conditions. An Atlantic salmon
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Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
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