Produced from liver tumours of DMH induced rats. Liver tumours were produced by intraportal injection of the parent line WB2054. This cell line forms liver tumours more readily than the parent line.
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
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