BRIN-BG5

10033001, rat pancreas (islets), Epitheloid

• Synonym(s): BRIN
• UNSPSC Code: 41106514

Properties

• product name: BRIN-BG5, 10033001
• biological source: rat pancreas (islets)
• growth mode: Adherent
• karyotype: Modal chromosome number 66 up to passage 50
• morphology: Epitheloid
• products: BRIN-BG5 is a stable, glucose-responsive insulin secreting beta cell line expressing insulin, glucokinase and IAPP as detected by immunocytochemistry. BRIN-BG5 cells express the GLUT2 glucose transporter.
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice

Description

Cell Line Origin

A rat pancreatic Beta cell: RINm5F hybrid cell line. Insulin-secreting cell line

Cell Line Description

BRIN-BG5 is a hybrid cell line formed by the electrofusion of a primary culture of NEDH rat pancreatic islets with RINm5F (a cell line derived from a NEDH rat insuliinoma). BRIN-BG5 has been shown to be tumourigenic when transplanted into a SCID mouse host. BRIN-BG5 has been mycoplasma eradicated at ECACC and the stocks available for supply have undergone a further 10 passages without detection of mycoplasma.

Culture Medium

RPMI 1640 (R0883) + 2 mM L-Glutamine (G7513) + FBS / FCS (F2442)

Subculture Routine

Split sub-confluent cultures (70-80%) 1:8 to 1:10, i.e. seeding at 2-4 x 10⁴ cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. Population doubling approximately28hrs. At confluence 105 cells/cm² can be expected.

Other Notes

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Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.