This cell line is a derivative of the Sf9 cell line which has been adapted to TiterHigh Sf Insect Medium (Sigma I5408). TiterHigh Sf Insect Medium is an animal component-free, protein-free formulation designed specifically for the Sf9 (Spodoptera frugiperda) insect cell line. The cell line Sf9 is derived from pupal ovarian tissue of spodoptera frugiperda. The Sf9 cell line is highly susceptible to Baculovirus infection and is used in the production of protein products genetically manipulated into Baculovirus vector systems.
Application
Virus studies: Baculovirus
Culture Medium
TiterHigh™ Sf Insect Medium (Sigma I5408) no supplements.
Subculture Routine
Maintain cultures between 3x100,000 to 1x1,000,000 cells/ml; 27°C. Gently resuspend cells in old culture medium by dislodging the cells by pipetting, or gentle agitation, and resuspend in fresh medium. Cells should stick to the culture flask. Freeze in 7.
Other Notes
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Legal Information
TiterHigh is a trademark of Sigma-Aldrich Co. LLC
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Eukaryotic cell lines aid in complex post-translational modifications and folding of recombinant proteins. Find suitable recombinant protein production cell lines.
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