C4018
Choloylglycine Hydrolase from Clostridium perfringens (C. welchii)
lyophilized powder, ≥100 units/mg protein
Synonym(s):
3α,7α,12α-Trihydroxy-5β-cholan-24-oylglycine amidohydrolase
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Pricing and availability is not currently available.
Recommended Products
form
lyophilized powder
Quality Level
specific activity
≥100 units/mg protein
composition
Protein, ≥50% biuret
shipped in
wet ice
storage temp.
−20°C
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Application
Biochem/physiol Actions
The enzyme is an N-terminal nucleophilic (Ntn) hydrolase that catalyzes the hydrolysis of amide bonds, liberates the glycine/taurine moiety from the steroid core and eventually yields unconjugated bile acids. Agents that oxidize thiol groups (e.g., p-mercuribenzoate, iodoacetamide, Hg2+, Cu2+, and Cd2+) have been shown to strongly inhibit bile salt hydrolase (BSH) activity in Clostridium perfringens.[4]
Unit Definition
One unit will hydrolyze 1.0 μmole of glycocholic acid to glycine and cholic acid in 5 min at pH 5.6 at 37 °C.
Physical form
Partially purified lyophilized powder containing buffer salts and stabilizer
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Effects of Quercetin and Rutin on Serum and Hepatic Lipid Concentrations, Fecal Steroid Excretion and Serum Antioxidant Properties
Nakamura Y, et al.
Journal of Health Science, 46(4), 229-240 (2000)
Bile salt hydrolase activity in probiotics.
Máire Begley et al.
Applied and environmental microbiology, 72(3), 1729-1738 (2006-03-07)
J M Lambert et al.
Journal of agricultural and food chemistry, 56(18), 8360-8364 (2008-08-30)
The interest in efficient intestinal delivery of health-promoting substances is increasing. However, the delivery of vulnerable substances such as enzymes requires specific attention. The transit through the stomach, where the pH is very low, can be detrimental to the enzymatic
Guohong Wang et al.
Journal of industrial microbiology & biotechnology, 38(8), 985-990 (2010-09-22)
Lactic acid bacteria (LAB) encounter various types of stress during industrial processes and gastrointestinal transit. Catalase (CAT) and bile salt hydrolase (BSH) can protect bacteria from oxidative stress or damage caused by bile salts by decomposing hydrogen peroxide (H(2)O(2)) or
S V Hosseini et al.
Journal of applied microbiology, 107(4), 1392-1403 (2009-05-12)
The characterization of four novel bacteriocin-producing enterococcal strains, isolated from nonfermented animal foods, was carried out with a view to evaluate their potential application as probiotics in raw and processed foodstuffs. 16S rRNA sequencing and random amplification of polymorphic DNA-polymerase
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