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C2730

Sigma-Aldrich

Cellulase from Trichoderma reesei

greener alternative

aqueous solution, ≥700 units/g

Synonym(s):

Celluclast®, Celluclast® 1.5L

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About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

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biological source

fungus (Trichoderma reesei)

Quality Level

form

aqueous solution

specific activity

≥700 units/g

mol wt

68 kDa

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

density

1.10-1.30 g/mL

greener alternative category

storage temp.

2-8°C

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General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research.

Cellulase from Trichoderma reesei is produced by submerged fermentation of a selected strain of the fungus Trichoderma reesei and catalyzes the breakdown of cellulose into glucose, cellobiose, and higher glucose polymers. It corresponds to a molecular mass of 68 kDa with optimum pH and temperature of 6 and 52° C, respectively.[1]

Application

Cellulase from Trichoderma reesei has been used:
  • for aqueous extraction of oil from corn germ[2]
  • in the enzymatic hydrolysis of Sweet Sorghum Bagasse (SSB)[3]
  • in the enzymatic digestion of paper grids[4]
Cellulase from Sigma has been used to degrade cello-oligosaccharides into glucose in order to investigate the biodegradability of bioabsorbable bacterial cellulose (BBC).[5]

Biochem/physiol Actions

Cellulase complexes with β-glucosidase for degrading cellobiose to glucose polymer.[6] The lignin content in straw and furfural residues hampers cellulase production.[7]

Legal Information

A product of Novozyme Corp.
Celluclast is a registered trademark of Novozymes Corp.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

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New insights into the enzymatic hydrolysis of lignocellulosic polymers by using fluorescent tagged carbohydrate-binding modules
Khatri V, et al.
Sustainable Energy & Fuels, 2(2), 479-491 (2018)
S Luti et al.
International journal of biological macromolecules, 165(Pt B), 2845-2854 (2021-03-20)
Cerato-platanin family (CPF) proteins are produced by fungi and elicit defences when applied to plants, behaving as PAMPs/MAMPs. CPF proteins share structural similarity to plant and bacterial expansins, and have been demonstrated, in some cases, to possess expansin-like loosening activity
Caoxing Huang et al.
Applied biochemistry and biotechnology, 180(8), 1508-1523 (2016-07-06)
In this work, to elucidate why the acid-pretreated bamboo shows disappointingly low enzymatic digestibility comparing to the alkali-pretreated bamboo, residual lignins in acid-pretreated and kraft pulped bamboo were isolated and analyzed by adsorption isotherm to evaluate their extents of nonproductive
Production of cellulase by Trichoderma reesei from pretreated straw and furfural residues
Zhao CH, et al.
Royal Society of Chemistry Advances, 8(63), 36233-36238 (2018)
Studies on the production and application of cellulase from Trichoderma reesei QM-9414
Krishna SH, et al.
Bioprocess Engineering, 22(5), 467-470 (2000)

Questions

1–8 of 8 Questions  
  1. What does the aqueous solution in which the cellulases are stored contain?

    1 answer
    1. While the specific formulation of this material is considered proprietary, this aqueous solution includes sodium chloride and potassium sorbate.

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  2. Is this product food grade and can be use for cellulosic part of a food material

    1 answer
    1. This product is not a food grade Cellulase from Trichoderma reesei.

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  3. What is the best buffer (including molarity & pH) for C2730 (cellulase)?

    1 answer
    1. The best buffer needed would depend on the application.

      Please see the link below to review the enzyme assay performed on the starting material for this product:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/352/413/c8546enz.pdf

      It may also be helpful to review the following literature:
      https://www.sciencedirect.com/science/article/pii/S2452014417300638#s0010
      https://www.sciencedirect.com/science/article/pii/S1096717608000396?via%3Dihub
      https://www.jstage.jst.go.jp/article/jpi/60/6/60_322/_pdf

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  4. Is any nuclease activity tested for the Cellulase (C2730) ?

    1 answer
    1. Cellulase (C2730) has not been tested for nuclease activity. Currently, there is not a cellulase product that is tested for nuclease, protease, DNAse, or RNAse.

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  5. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  6. What is the protein concentration of Product C2730, Cellulase?

    1 answer
    1. The protein concentration for cellulase (C2730) is not determined.  The density of this cellulase solution is approximately 1.2 g/mL.

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  7. What are the optimal conditions for Product C2730, Cellulase?

    1 answer
    1. For cellulase (C2730) the optimal pH range is 4.5 - 6.0, and the optimal temperature range is 50 - 60°C.

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  8. What is the unit definition for the activity of Product C2730, Cellulase?

    1 answer
    1. An EGU (Endoglucanase Unit) is measured relative to a Novozyme cellulase standard. The assay utilizes carboxymethyl cellulose (CMC) as the substrate and the assay is performed at 40°C. at pH 6.0.

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