C-12665
Human Renal Epithelial Cells (HREpC)
500,000 cryopreserved cells
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About This Item
UNSPSC Code:
41106514
NACRES:
NA.81
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biological source
human kidney
packaging
pkg of 500,000 cells
morphology
(epithelial)
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Related Categories
General description
Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.
Cell Line Origin
Kidney
Application
Primary Human Renal Epithelial Cells (HREpC) are isolated from the adult kidney and are provided in a cryopreserved format. HREpC stain positive for cytokeratin.Kidneys have a very complex structure and contain diverse epithelia such as those in the convoluted tubules, the cortical collecting ducts, the calyxes, and the renal pelvis. The epithelial cells present in the distinct ducts and tubules differ in their morphology and physio-logical function. Consequently, HREpC are comprised of a heterogeneous population of epithelial cells. Important physiological processes like osmoregulation and excretion can be studied with HREpC in vitro.
Quality
Rigid quality control tests are performed for each lot of Epithelial Cells. They are tested for cell morphology, adherence rate and cell viability. Furthermore, flow cytometric analyses for the cell-type specific marker cytokeratin are carried out for each lot. Growth performance is tested through multiple passages under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).
Warning
Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.
Subculture Routine
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Other Notes
Recommended Plating Density: 10000 - 15000 cells per cm2Passage After Thawing: P2Tested Markers: Cytokeratin positiveGuaranteed population doublings: >15
Recommended products
Recommended Primary Cell Culture Media:Link
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Articles
PromoCell offers optimized primary human renal epithelial cells and growth medium with protocols for handling and cultivation.
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