B0555
Anti-Vascular Endothelial Growth Factor Receptor-2 (KDR)−Biotin Conjugate antibody, Mouse monoclonal
clone 260.4, KDR-2, purified from hybridoma cell culture
Synonym(s):
Monoclonal Anti-Vascular Endothelial Growth Factor Receptor-2 antibody produced in mouse, Anti-KDR, Anti-VEGFR-2
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About This Item
UNSPSC Code:
51111800
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
conjugate
biotin conjugate
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
260.4, KDR-2, monoclonal
form
buffered aqueous solution
species reactivity
human
technique(s)
western blot: 1:1,000 using insect cell culture supernatant expressing human extracellular recombinant VEGF R
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... KDR(3791)
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General description
Biotin Conjugated Monoclonal Anti-VEGF Receptor-2 (KDR) (mouse IgG1 isotype) is derived from the KDR-2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a purified recombinant human extracellular VEGF receptor-2 (KDR). Vascular endothelial growth factor (VEGF) is also called vasculotropin (VAS), vascular permeability factor (VPF) and kinase-insert domain containing receptor (KDR8). It is a member of a family of endothelial cell mitogens and angiogenic factors. VEGF is a homodimeric heparin-binding glycoprotein. The KDR gene has been mapped to human chromosome 4q11-q12. The homologous gene for the human KDR gene is mouse Flk1 (fetal liver kinase 1) and rat TKrc.
Specificity
Monoclonal Anti-Vascular Endothelial Growth Factor Receptor-2 (KDR)-Biotin Conjugate antibody is purified from ascites fluid, and conjugated withSulfo-NHS-LC-Biotin. It is specific for human VEGF receptor2. It does not cross react with Flt1, Flt4 or platelet-derived growth factor receptor β (PDGF-R1).
Reacts specifically with human VEGF receptor-2. This clone has also been termed 260.4. It does not recognize Flt1 (VEGFR-1), Flt4 (VEGFR-3), or platelet-derived growth factor receptor (PDGF-R).
Immunogen
purified recombinant human extracellular VEGF receptor-2 (KDR).
Application
Monoclonal Anti-Vascular Endothelial Growth Factor Receptor-2 (KDR)-Biotin Conjugate antibody produced in mouse has also been used in :
- enzyme linked immunosorbent assay (ELISA)
- immunoblotting
- flow cytometry
- immunohistochemistry
- immunohistochemistry
Biochem/physiol Actions
Vascular endothelial growth factors receptors (VEGF Rs) are receptor tyrosine kinases that bind to VEGF that plays important role in embryonic vasculogenesis, angiogenesis and homeostasis. VEGF R2 has the ability to form heterodimers with VEGF R1 and VEGF R3. The binding of VEGF to VEGF R2 results in activation of tyrosine amino acids of the cytoplasmic tail of the receptor. This interaction results in the activation of MAPK, PI3K, PKC, FAK and Src kinase pathways. The biological roles of VEGF R2 include in epithelial cell growth, proliferation, survival, migration and vascular permeability and angiogenesis. Due to its role in promoting migration and angiogenesis, VEGF R2 has been implicated in breast and prostate cancer.
Target description
The KDR receptor (VEGFRa), defining marker of hematopoietic stem cells (HSCs), distinguishes pluripotent HSCs from lineage-committed progenitor HSCs.
Physical form
Solution in phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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T Böldicke et al.
Stem cells (Dayton, Ohio), 19(1), 24-36 (2001-02-24)
Five specific single-chain antibodies recognizing the human vascular endothelial growth factor receptor-2 (VEGFR-2/KDR) were selected from a V-gene phage display library constructed from mice immunized with the extracellular domain of VEGFR-2 (Ig-like domain 1-7). All five scFv antibodies (A2, A7
Shanchun Guo et al.
Biochimica et biophysica acta, 1806(1), 108-121 (2010-05-14)
Investigations over the last decade have established the essential role of growth factors and their receptors during angiogenesis and carcinogenesis. The vascular endothelial growth factor receptor (VEGFR) family in mammals contains three members, VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1) and VEGFR-3 (Flt-4)
P Berger et al.
Swiss medical weekly, 141, w13318-w13318 (2011-12-20)
Vascular endothelial growth factors (VEGFs) regulate blood and lymph vessel formation through activation of the type V receptor tyrosine kinases VEGFR-1, -2 and -3. In addition, VEGFs interact with co-receptors such as neuropilins, integrins, semaphorins or heparansulfate glycosaminoglycans. Ligand binding
Hyperoxia reduces bone marrow, circulating, and lung endothelial progenitor cells in the developing lung: implications for the pathogenesis of bronchopulmonary dysplasia
Balasubramaniam V, et al.
American Journal of Physiology. Lung Cellular and Molecular Physiology, 292(5), L1073-L1084 (2007)
Role of vascular endothelial growth factor in regulation of physiological angiogenesis
Ferrara N
American Journal of Physiology. Cell Physiology, 280(6), C1358-C1366 (2001)
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