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I8377

Sigma-Aldrich

Iodonitrotetrazolium chloride

Used in colorimetric assays.

Synonym(s):

2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride, p-Iodonitrotetrazolium Violet, INT

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250 MG
RM 665.00
1 G
RM 1,572.00
5 G
RM 6,667.00
25 G
RM 30,185.00

RM 665.00


Estimated to ship on06 April 2025


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250 MG
RM 665.00
1 G
RM 1,572.00
5 G
RM 6,667.00
25 G
RM 30,185.00

About This Item

Empirical Formula (Hill Notation):
C19H13ClIN5O2
CAS Number:
Molecular Weight:
505.70
Beilstein:
4093224
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

RM 665.00


Estimated to ship on06 April 2025


Request a Bulk Order

Quality Level

Assay

≥98% (TLC)

form

powder

mp

240 °C (dec.) (lit.)

solubility

H2O: 4 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

[Cl-].[O-][N+](=O)c1ccc(cc1)-[n+]2nc(nn2-c3ccc(I)cc3)-c4ccccc4

InChI

1S/C19H13IN5O2.ClH/c20-15-6-8-16(9-7-15)23-21-19(14-4-2-1-3-5-14)22-24(23)17-10-12-18(13-11-17)25(26)27;/h1-13H;1H/q+1;/p-1

InChI key

JORABGDXCIBAFL-UHFFFAOYSA-M

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Application

Electron acceptor for the colorimetric assay of various dehydrogenases
Iodonitrotetrazolium chloride has been used for the staining of cells.[1] It has been used as a coupling agent in the enzymatic assay of D-arabinitol.[2]

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Pictograms

FlameExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Flam. Sol. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

4.1B - Flammable solid hazardous materials

WGK

WGK 3


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Constitutive activation of pp125fak in newly isolated human breast cancer cell lines.
Ignatoski KMW and Ethier SP
Breast Cancer Research and Treatment, 54, 173-173 (1999)
Sergio Ravasio et al.
Biochemistry, 41(25), 8120-8133 (2002-06-19)
The properties of the recombinant ferredoxin-dependent glutamate synthase of Synechocystis PCC6803 were determined by means of kinetic and spectroscopic approaches in comparison to those exhibited by the bacterial NADPH-dependent enzyme form. The ferredoxin-dependent enzyme was found to be similar to
Yang Hongwei et al.
Ecotoxicology and environmental safety, 59(2), 249-255 (2004-08-26)
Anaerobic biodegradability of organic compounds can be assessed from the changes in organic compounds, the end products, and the activity of microorganisms. In this study, all three of these variables were considered to integrate assessment of the anaerobic biodegradability of
Xiao-mei Xie et al.
Journal of environmental sciences (China), 16(2), 252-255 (2004-05-13)
The impact of triazophos, bensulfuron-methyl, chlobenthiazone on soil biochemical characteristics in a paddy soil under controlled moisture (flooded soil) and temperature (25 degrees C) condition was studied. The electron transport system (ETS)/dehydrogenase activity displayed a negative correlation with triazophos, bensulfuron-methyl
F Mermillod-Blondin et al.
Water research, 39(9), 1687-1698 (2005-05-19)
Biogeochemical processes mediated by microorganisms in river sediments (hyporheic sediments) play a key role in river metabolism. Because biogeochemical reactions in the hyporheic zone are often limited to the top few decimetres of sediments below the water-sediment interface, slow filtration

Questions

1–6 of 6 Questions  
  1. Is there a procedure for reducing Product I8377, Iodonitrotetrazolium chloride, by superoxide radicals?

    1 answer
    1. Yes.  See Biochem Biophys Res Commun. 1988 Feb 15;150(3):1294-301.

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  2. Product I8377, Iodonitrotetrazolium chloride, is stated to have solubility in water, ethanol and ethylene glycol monomethylether.  What other solutions can be used?

    1 answer
    1. Conn's Biological Stains, 10th Edition (page 163) states P-Iodonitrotetrazolium Violet is also soluble in dimethylsulfoxide and dimethylsulfoxide-water mixtures.

      Helpful?

  3. Can Product I8377, Iodonitrotetrazolium chloride, be used for the staining of cells?

    1 answer
    1. Yes, although due to the large crystal size of the formazan and lack of substantivity, histological application of I8377 has been limited.  In microbiology, the product was used by Lussenhop and Fogel (1993) for in situ microscopic observation of soil biota.. Gribbon and Barer (1995) used for the demonstration of viable but nonculturable bacteria.  Heidelberg (1997) used as a bacterial viability stain.

      Helpful?

  4. Is Product I8377, Iodonitrotetrazolium chloride, lipophilic?

    1 answer
    1. Yes, I8377  is a lipophilic cation of moderate size.  The resulting formazan is more strongly liophilic and fat soluble.

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  5. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  6. Is there a reference for using Product I8377, Iodonitrotetrazolium chloride, to treat brain membrane preparations?

    1 answer
    1. Yes.  See Neuroscience Letters Volume 35, Issue 2, 21 February 1983, Pages 215-220

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