XTG360-RO

X-tremeGENE^™ 360 Transfection Reagent

Universal polymer reagent for delivering DNA, siRNA, miRNA and CRISPR/RNP to many cell lines

• Synonym(s): transfection reagent
• UNSPSC Code: 41106502
• NACRES: NA.55

Properties

• grade: for molecular biology
• form: liquid (aqueous solution)
• packaging: pkg of 0.4 mL (08724105001); pkg of 1.0 mL (08724121001); pkg of 5 × 1.0 mL (08724156001)
• manufacturer/tradename: Roche
• technique(s): transfection: suitable
• storage temp.: −20°C

Description

General description

X-tremeGENE^™ 360 Transfection Reagent is an innovative reagent that forms a complex with DNA or RNA and efficiently delivers the nucleic acids into animal or insect cells. With improved utility, X-tremeGENE^™ 360 delivers high efficiency transfection in more cell types with low cytotoxicity.

Application

X-tremeGENE^™ 360 Transfection Reagent is a high performance transfection reagent, free of animal-derived components.

• Designed to transfect a broad range of eukaryotic cells, many cell lines not transfected well by other reagents, and hard-to-transfect cell lines, such as asK-562 and HepG2.
• Can be successfully used in a variety of applications, such as gene expression analysis using transiently transfected cells, generating of stable cell lines, expression of siRNA for gene knockdown studies, and CRISPR gene-editing tools.
• Produces minimal cytotoxicity or changes in morphology when adequate numbers of cells are transfected, eliminating the requirement to change media after adding the transfection complex.
• Suitable for transient and stable transfection.
• Functions well in the presence or absence of serum.

Features and Benefits

X-tremeGENE^™ 360 Transfection Reagent is a high-performing, versatile and reliable solution for hard-to-transfect and common cell types. As a proven and reliable reagent, X-tremeGENE^™ 360 allows researchers to avoid guesswork when performing transfection. In addition, X-tremeGENE^™ 360 exhibits:

• Peak performance in a broad range of cell types
• Suitable for multiple applications
• Efficient delivery of plasmid DNA, siRNA/miRNA and CRISPR/Cas9 components

Quality

Each lot of X-tremeGENE^™ 360 Transfection Reagent is carefully tested following established quality procedures to ensure that the product is consistently performing according to specifications. During quality testing, cells are transfected with a reporter gene vector DNA using X-tremeGENE^™ 360 Transfection Reagent (ratio 3:1 μL/μg DNA). Reporter gene activity is monitored via chemiluminescent detection. Using a standard curve, the total amount of recombinant protein is determined per well in order to meet specification.

Physical form

Supplied in 80% ethanol and sterile-filtered through 0.2 μm pore size membrane. Does not contain any ingredients of human or animal origin. Number of Tests: Using the standard procedure, 1 mL of X-tremeGENE^™ 360 Transfection Reagent can be used to perform up to 10,000 transfections in 96-well plates.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

X-tremeGENE is a trademark of Roche

Safety Information

• Pictograms: GHS02,GHS07
• Signal Word: Danger
• Hazard Statements: H225 - H319
• Precautionary Statements: P210 - P233 - P280 - P303 + P361 + P353 - P337 + P313 - P370 + P378
• Hazard Classifications: Eye Irrit. 2 - Flam. Liq. 2
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 1
• Flash Point(F): 61.7 °F
• Flash Point(C): 16.5 °C