11636111910

PCR ELISA, DIG-Detection

• UNSPSC Code: 41116133

Properties

• usage: sufficient for 192 detection reactions (two microplates)
• manufacturer/tradename: Roche
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

PCR ELISA, DIG-Detection kit is used for the semi-quantitative detection of digoxigenin (DIG)-labeled polymerase chain reaction (PCR) products. We recommend to use the PCR ELISA, DIG-Detection kit in combination with the PCR ELISA, DIG-Labeling kit.

Specificity

The high specificity of digoxigenin detection permits distinguishing specific and nonspecific amplification products. This is due to the hybridization step with the capture probe. The specificity of this hybridization follows the standard rules of hybridization assays. Stringent hybridization in the digoxigenin detection depends on the length and the GC content of the capture probe and the incubation temperature. Temperatures up to 55 °C are compatible with digoxigenin detection. Under these conditions, with short capture probes (18 to 20mers), identification of a single base mismatch in the capture oligonucleotide is possible. For this reason, mutations in template DNAs can also be detected using DIG.

Application

The PCR-ELISA DIG detection kit has been used for the detection of Shiga toxin-producing E. coli in food.

Packaging

1 kit containing 11 components.

Specifications

This kit is part of a complete PCR ELISA system. If you combine it with other reagents and equipment, you can adapt the system for many molecular biology applications. For example:

• If you have an appropriate target-specific, biotin-labeled capture probe, you can distinguish PCR products that differ by as little as a single base pair. Thus the PCR ELISA system (DIG-labeling + DIG-detection) can be used:

to detect point mutations, deletions or insertions.
to classify target sequences (e.g., as in HLA-typing or cell typing).

• Preparation of suitable DIG-labeled standards and addition of a colorimetric detection system will allow you to quantify PCR products.
• The microplate format used by the PCR ELISA system makes the system compatible with automated plate preparation and reading systems.

Labeling: The DIG-labeled PCR products detected with this kit contain alkali-stable digoxigenin-11-dUTP (DIG-dUTP). Such DIG-labeled products can be prepared with the PCR ELISA, DIG-Labeling kit.
Capacity: This size of the PCR ELISA, DIG-Detection kit allows semi-quantitative detection of approx. 50 DIG-labeled PCR products. This number will vary depending upon the number of sample dilutions, controls, and/or standards used in the assay.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit Components Only

• Control PCR Product, digoxigenin-labeled, lyophilizate, sufficient for five control reactions (three dilutions each)
• Control Capture Probe, biotin-labeled, lyophilizate, sufficient for five control reactions (three dilutions and one negative control each)
• Denaturation Solution (contains NaOH) ready-to-use
• Hybridization Buffer ready-to-use
• Washing Buffer Tablets
• Conjugate Dilution Buffer ready-to-use
• Anti-Digoxigenin-peroxidase-conjugate antibody, stabilized lyophilizate
Substrate Buffer ready-to-use
ABTS Tablets
Microplate Modules (8 wells each in a strip frame), precoated with streptavidin and postcoated with Blocking reagent; shrink-wrapped with a desiccant capsule.
Cover Foils for microplates

See All (11)

Safety Information

• Pictograms: GHS05,GHS07,GHS09
• Signal Word: Danger
• Hazard Statements: H317,H318,H411
• Precautionary Statements: P261 - P273 - P280 - P305 + P351 + P338 + P310 - P333 + P313 - P391
• Hazard Classifications: Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): does not flash
• Flash Point(C): does not flash