04693159001
Roche
cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail
Tablets provided in EASYpacks
Synonym(s):
Protease Inhibitor Tablets
About This Item
Recommended Products
form
tablet
packaging
pkg of 30 tablets (individually packed in foil blister packs)
manufacturer/tradename
Roche
concentration
(Starting concentration: 1 tablet contains protease inhibitors sufficient for a 10 ml cell extract.)
technique(s)
inhibition assay: suitable
solubility
aqueous buffer: soluble
water: soluble
absorption
0.08 at 280 nm
storage temp.
2-8°C
Related Categories
General description
Due to the optimized composition of the tablets they show excellent inhibition of serine and cysteine proteases, and are well suited for the protection of proteins isolated from animal tissues, plants, yeast, and bacteria. cOmplete, Mini, EDTA-free tablets, contains both irreversible and reversible protease inhibitors. Metallo- and aspartic proteases are not inhibited.
cOmplete Mini, EDTA-free Tablets are identical to cOmplete Mini Tablets, the only difference being that no EDTA or other chelating (e.g. EGTA) agents are included.
Specificity
Application
- nonidet P-40 (NP-40) buffer for the preparation of cell lysate of neuroblastoma cells
- tissue lysis buffer for homogenisation and lysis of prefrontal cortex tissues
- ice-cold radioimmunoprecipitation assay (RIPA) buffer for the lysis of Chinese hamster ovary (CHO-K1) cells
cOmplete, Mini, EDTA-free Tablets, are used for the inhibition of proteolytic activity in small volumes (up to 10 ml) in which EDTA may interfere with protein stability (e.g., metal-containing proteins) or subsequent assays. Since EDTA interferes with IMAC (Immobilized Metal Affinity Chromatography), cOmplete, Mini, EDTA-free, is preferentially used in the isolation process of Poly-His-tagged fusion proteins.
If it is necessary to inhibit proteolytic activity in a large volume (up to 50 ml), we recommend to use cOmplete, EDTA-free.
Features and Benefits
- Convenience: Simply push the quick-dissolving tablet through the foil packaging of the EASYpack into your buffer.
- Complete protection: Instantly protect your proteins against a broad range of proteases (see Table 1).
- Flexible: Protect proteins in extracts from almost any tissue or cell, including animals, plants, yeast, bacteria, or fungi.
- Safe: Choose non-toxic inhibitors that pose no risk to you or those around you.
Packaging
Quality
Physical form
Preparation Note
Working solution: Handling
Carefully push the tablet through the foil packaging using the base of your thumb (not fingernail) to prevent the breakage of tablets.
Preparation of Working Solution
One cOmplete Mini EDTA-free tablet is sufficient for the inhibition of the proteolytic activity in 10 ml extraction solution. When very high proteolytic activity is present, one tablet should be used for 7 ml extraction buffer. Tablets can be added directly to the extraction medium. Alternatively a stock solution (7 X conc.) can be prepared.
Stock solution (7 X conc.)
Dissolve one cOmplete Mini EDTA-free tablet in 1.5 ml dist. water or in 1.5 ml 100 mM phosphate buffer, pH 7.0.
Storage conditions (working solution): The stock solution can be stored at 2 to 8 °C for 1 to 2 weeks, or at least 12 weeks at -15 to -25 °C.
Reconstitution
Storage and Stability
Other Notes
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.
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