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UFSC40001

Amicon® Stirred Cell 400mL

This Amicon stirred cell model provides high flow rates with solutions up to 10% macrosolute concentration. Capable of rapid concentration, or salt removal followed by concentration in the same unit.

Synonym(s):

Amicon stirred cell

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About This Item

UNSPSC Code:
41104903
eCl@ss:
32031690
Pricing and availability is not currently available.

material

Nylon assembly (cap and tube fitting)
Nylon stand (retaining)
Tygon® tube (filtrate)
anodized aluminum stand (retaining)
polyacetal assembly (stirring)
polyethylene tube (pressure)
polysulfone assembly (stirring)
polysulfone holder (cylinder & membrane)
silicone O-ring

Quality Level

product line

Amicon®

manufacturer/tradename

Amicon®

parameter

400 mL process volume

technique(s)

protein clean-up: suitable (desalting)
protein purification: suitable (concentration)

H

18.7 cm (7.4 in.)

filter diam.

76 mm

filtration area

41.8 cm2

shipped in

ambient

Application

This Amicon stirred cell model provides high flow rates with solutions up to 10% macrosolute concentration. Capable of rapid concentration, or salt removal followed by concentration in the same unit.

Principle

Pressure

Linkage

Replaces: 5124

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
Tygon is a registered trademark of Saint-Gobain Performance Plastics Corp.

Certificates of Analysis (COA)

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Articles

Amicon Stirred Cells concentrate and buffer-exchange macrosolutes efficiently with pressure-driven filtration.

Questions

1–3 of 3 Questions  
  1. Does this model have a 10 kDa membrane?

    1 answer
    1. The 10 kDa membrane suitable for this stirred cell is PLGC07610.

      Helpful?

  2. What is the minimum volume that can be concentrated using stirred cells? Additionally, what is the minimum volume that can be recovered from the stirred cell?

    1 answer
    1. The stirred cells do not have a specified minimum concentrate volume, as this is typically dependent on the sample. According to the user guide, the minimum concentrate volume must be determined empirically by the user based on sample composition and stirring speed.

      In theory, it is possible to concentrate to dryness; however, this is highly dependent on the sample and membrane used.

      Additionally, the specification chart includes a hold-up volume, which indicates the volume of sample that is 'lost' to the device. For detailed hold-up volume information, please refer to page 10 of the user guide.

      Helpful?

  3. Could you please provide more information about the troubleshooting for a stirred cell that is not holding pressure and has poor concentrate recovery?

    1 answer
    1. On September 7, 2021, the Eyre User Guide provided troubleshooting information on pages 8 and 9. It addressed the symptom of a stirred cell not holding pressure and poor concentrate recovery. The cause identified was a membrane that was not sealed, leading to sample bypass around the membrane. The corrective action recommended was to ensure that the O-ring is free of dirt or debris and undamaged.

      Helpful?

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