Lymphocyte activation gene 3 protein (UniProt P18627; also known as CD223, LAG-3, Protein FDC) is encoded by the LAG3 (also known as FDC) gene (Gene ID 3902) in human. LAG3 is a single-pass type I membrane protein that binds MHC class II molecules on antigen presenting cells with high affinity. LAG3 expression is induced after T cell activation and functions as a negative regulator of T cell function. LAG3 blockage is shown to augment T cell proliferation and cytokine production, and LAG3-deficient mice have a defect in the downregulation of T cell responses induced by staphylococcal enterotoxin B or by virus infection. In addition, LAG3 is expressed on activated regulatory T (Treg) cells at higher levels than on activated effector T cells. LAG3 blockage inhibits the suppressive activity of Treg cells. LAG3 is also upregulated on tumour-infiltrating lymphocytes (TILs), and LAG3 blockage can enhance anti-tumour T cell responses.
Immunogen
Mouse Fc-tagged recombinant human LAG3 extracellular fragment.
Application
Flow Cytometry Analysis: A 1:100 dilution of this antibody from a representative lot detected LAG3 in 1X10E6 Jurkat cells subjected to CD3/CD28 costimulation.
This Anti-LAG3 Antibody, clone 8C7.1 is validated for use in Western Blotting, Flow Cytometry for the detection of LAG3.
Quality
Evaluated by Western Blotting in NK-92c ell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected LAG3 in 10 µg of NK-92 cell lysate.
Concentration: Please refer to lot specific datasheet.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Certificates of Analysis (COA)
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