MABF2815

Anti-SARS-CoV-2 Spike RBD Antibody, clone 6C2-G9

• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 6C2-G9, monoclonal
• mol wt: calculated mol wt 141.18 kDa; observed mol wt ~200 kDa
• species reactivity: virus
• packaging: antibody small pack of 100 μg
• technique(s): ELISA: suitable; immunoprecipitation (IP): suitable; neutralization: suitable; western blot: suitable
• isotype: IgG1
• UniProt accession no.: P0DTC2
• shipped in: dry ice
• storage temp.: -10 to -25°C
• target post-translational modification: unmodified

Description

General description

Spike glycoprotein (UniProt: P0DTC2; also known as S glycoprotein, E2, Peplomer protein) is encoded by the S (also known as 2) gene (Gene ID: 43740568) in Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The SARS-CoV-2 is a positive-strand RNA virus that causes severe respiratory syndrome in human. The mature SARS-CoV-2 contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S). E and M proteins help in viral assembly and N protein is needed for RNA synthesis. The S protein is a single-pass type I, homotrimeric, membrane glycoprotein that is responsible for virus binding and entry into host cell. It is synthesized with a signal peptide (aa 1-12), which is subsequently cleaved off to generate the mature protein that contains an extracellular domain (aa 13-1213), a transmembrane domain (aa 1214-1234), and a cytoplasmic domain 1235-1273). The S protein is further processed into S1 (aa 13-685) and S2 (aa 686-1273) subunits by host cell furin. The presence of a furin polybasic cleavage site in S protein from SARS-CoV-2 sets it apart from S protein in SARS-CoV that possesses a monobasic S1/S2 cleavage site. The S1 subunit has the receptor binding domain (RBD; aa 319-541) that mediates entry of SARS-CoV-2 into sensitive cells through the peptidase domain of host Angiotensin-converting enzyme 2 (ACE2) with high affinity (KD = 15 nM). The S2 protein, which is reported to be well conserved, is responsible for membrane fusion. Clone 6C2-G9 binds to RBD of the SARS-CoV-2 spike protein.

Specificity

Clone 6C2-G9 is a mouse monoclonal antibody that detects the receptor binding domain (RBD) in the spike protein of Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).

Immunogen

His-tagged recombinant fragment corresponding to the receptor binding domain (319-541) within the spike protein of Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).

Application

Quality Control Testing

Evaluated by Western Blotting in lysate from HEK293T cells expressing full-length spike protein of SARS-CoV-2 virus.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Spike glycoprotein in HEK293T cells expressing full-length spike protein of SARS-CoV-2, but not in lysate from wild-type HEK293T cells.

Tested Applications

Neutralizing: A representative lot neutralized SARS-CoV-2 Spike protein RBD to ACE2 in Neutralizing applications (Courtesy of Kento Abe and Anne-Claude Gingras, Toronto).

ELISA Analysis: A 1:3 dilution from a representative lot detected SARS-CoV-2 Spike protein. (Courtesy of Stefan Schüchner, Ingrid Mudrak, Ingrid Frohner, and Egon Ogris (Max Perutz Labs, Medical University of Vienna, Austria).

Western Blotting Analysis: A 1:400 dilution from a representative lot detected Sars-CoV-2 Spike protein in Whole cell lysates of HEK293T cells expressing full-length spike protein of SARS-CoV-2. (Courtesy of Stefan Schüchner, Ingrid Mudrak, Ingrid Frohner, and Egon Ogris (Max Perutz Labs, Medical University of Vienna, Austria).

Neutralizing: A representative lot neutralized SARS-CoV-2 Spike protein RBD binding to ACE2 receptor. (Abe, K.T., et al. (2020). JCI Insight 5(19):e142362).

Immunoprecipitation Analysis: A representative lot immunoprecipitated SARS-CoV-2 Spike protein. (Courtesy of Stefan Schüchner, Ingrid Mudrak, Ingrid Frohner, and Egon Ogris (Max Perutz Labs, Medical University of Vienna, Austria).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-SARS-CoV-2 Spike RBD, clone 6C2-G9, Cat. No. MABF2815, is a mouse monoclonal antibody that detects Spike glycoprotein of SARS-CoV-2 and is tested for use in ELISA, Immunoprecipitation, Neutralizing, and Western Blotting.

Physical form

Purified mouse monoclonal antibody IgG1 in PBS without azide.

Storage and Stability

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable