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MAB1334

Sigma-Aldrich

Anti-Collagen Types I, II & III Antibody, clone MMCHABC

clone MMCHABC, Chemicon®, from mouse

Synonym(s):

Interstitial Collagens

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

MMCHABC, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable
radioimmunoassay: suitable

isotype

IgM

suitability

not suitable for Western blot

NCBI accession no.

NM_000088.3

UniProt accession no.

P02452
P02458
P02461

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... COL1A1(1277)

Specificity

Human collagen types I, II and III (both native and denatured). No cross reactivity with human plasma proteins, other collagen types or non-collagenous proteins in ELISA, RIA or immunoblot.

Immunogen

Human collagen type I

Application

Anti-Collagen Types I, II & III Antibody, clone MMCHABC detects level of Collagen Types I & has been published & validated for use in ELISA, RIA, IH.
ELISA: Apparent binding constant from titration curves is 0.1 nM. Can be used as capture or reporter antibody for determination of collagen I or III in sandwich ELISA, but requires combination with collagen type-specific antibody for other function.

Immunohistochemistry: cryostat sections fixed with acetone or ethanol. Staining pattern similar to polyclonal antibody to interstitial collagens. Formaldehyde fixation is not recommended.

Not suitable for use on paraffin embedded sections or immunoblots.

Optimal working dilution must be determined by the end user.
Research Category
Cell Structure
Research Sub Category
ECM Proteins

Physical form

Format: Purified
Purified from murine ascites by PEG precipitation and Sepharose™ CL-4B chromatography. Lyophilized Powder.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Sepharose is a trademark of Cytiva

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H315,H319,H360FD

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Gloria Fabris et al.
Biophysical journal, 115(9), 1770-1782 (2018-10-17)
Basement membranes (BMs) are thin layers of condensed extracellular matrix proteins serving as permeability filters, cellular anchoring sites, and barriers against cancer cell invasion. It is believed that their biomechanical properties play a crucial role in determining cellular behavior and

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