CC117
Chicken Extracellular Chondroitin Sulfate Proteoglycans
Synonym(s):
Chicken Chondroitin Proteoglycans, Chondroitin Sulfate Proteoglycans, Extracellular Proteoglycans
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About This Item
UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.75
Recommended Products
biological source
chicken
Quality Level
100
300
Assay
95%
form
liquid
mol wt
<400 kDa
manufacturer/tradename
Chemicon®
concentration
1 mg/mL
technique(s)
cell culture | mammalian: suitable
input
sample type hematopoietic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type epithelial cells
sample type pancreatic stem cell(s)
sample type neural stem cell(s)
sample type mesenchymal stem cell(s)
shipped in
dry ice
storage temp.
−20°C
Gene Information
chicken ... VCAN(395565)
General description
Aggrecan, versican, and neurocan are members of the family of hyaluronic acid-binding proteoglycans and therefore each contains hyaluronic acid binding regions, Ig-like domains, EGF like domains, lectin-like domains, and complement regulatory-like domains. Phosphacan is distinct; it contains a region of similarity to carbonic anhydrase, a fibronectin type III domain, and is a splice variant of the transmembrane protein receptor tyrosine phosphatase equivalent to its extracellular portion.
Although aggrecan was traditionally thought of as a cartilage proteoglycan and neurocan and phosphacan were thought of as neural proteoglycans, recent studies indicate that all four of these proteoglycans have broad yet distinctive distribution patterns inearly embryos. The presence of these proteoglycans has not been carefully examined in adult tissue beyond the expected sites. These proteoglycans are anti-adhesion molecules and are capable of inhibiting both cell-ECM and cell-cell adhesion.
Although aggrecan was traditionally thought of as a cartilage proteoglycan and neurocan and phosphacan were thought of as neural proteoglycans, recent studies indicate that all four of these proteoglycans have broad yet distinctive distribution patterns inearly embryos. The presence of these proteoglycans has not been carefully examined in adult tissue beyond the expected sites. These proteoglycans are anti-adhesion molecules and are capable of inhibiting both cell-ECM and cell-cell adhesion.
DESCRIPTION/COMPOSITION:CC117 contains a mixture of large, extracellular chondroitin sulfateproteoglycans isolated from embryonic chicken brain. Amino acid sequence analyses and immunological studies have demonstrated that the major components of this mixture are neurocan, phosphacan, versican, and aggrecan. Following chondroitinase treatment to remove the glycosaminoglycan portion of these proteoglycans, the major components of this preparation resolved on SDS gels are 250 kDa core proteins representing neurocan and 400 kDa core proteins representing aggrecan, phosphacan, and versican.
*Quantity: Protein as determined using the bicinchoninic acid (BCA) assay. Note that 100 μg is a measurement of core polypeptides. Components of this mixture contain core polypeptide plus attached glycosaminoglycans
*Quantity: Protein as determined using the bicinchoninic acid (BCA) assay. Note that 100 μg is a measurement of core polypeptides. Components of this mixture contain core polypeptide plus attached glycosaminoglycans
Product Source: Isolated from whole 12-14 day old embryonic chicken brains.
Application
CC117 has been used successfully with rat and mouse brain slice culture systems.
Physical form
Provided as a liquid in PBS, containing no preservatives.
Storage and Stability
Store at -20°C for up to 12 months. Avoid repeated freeze/thaw.
Analysis Note
Mixture of large, extracellular chondroitin sulfate proteoglycans (CSPGs) purified from embryonic chicken brains by chromatrographic methods. By PAGE analysis material is 95% pure, and migrates as a large HMW (<400kDa) mass under reduced conditions. The precise amounts of each CSPG contained in the mixture is not quantified, however the virtually all of the CSPGs present in the source brains are collected.
HOMOGENEITY:
By PAGE analysis material is 95% pure, and migrates as a large HMW (<400kDa) mass under reduced conditions. The precise amounts of CSPG species has not been determined.
HOMOGENEITY:
By PAGE analysis material is 95% pure, and migrates as a large HMW (<400kDa) mass under reduced conditions. The precise amounts of CSPG species has not been determined.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Find documentation for the products that you have recently purchased in the Document Library.
E Förster et al.
Development (Cambridge, England), 128(15), 3029-3039 (2001-09-05)
In various brain regions, particularly in the hippocampus, afferent fiber projections terminate in specific layers. Little is known about the molecular cues governing this laminar specificity. To this end we have recently shown that the innervation pattern of entorhinal fibers
H Ernst et al.
Journal of cell science, 108 ( Pt 12), 3807-3816 (1995-12-01)
Chondroitin sulfate proteoglycans inhibit the adhesion of cells to extracellular matrix proteins that otherwise permit adhesion. Although proteoglycans are widely assumed to act by masking the other protein in a mixed substrate, recent studies suggest that proteoglycans inhibit adhesion through
Keratan sulfate proteoglycan phosphacan regulates mossy fiber outgrowth and regeneration.
Butler, Christy D, et al.
The Journal of Neuroscience, 24, 462-473 (2004)
The interaction of the retina cell surface N-acetylgalactosaminylphosphotransferase with an endogenous proteoglycan ligand results in inhibition of cadherin-mediated adhesion.
Balsamo, J, et al.
The Journal of cell biology, 129, 1391-1401 (1995)
Jinyoung Lee et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(27), 15955-15966 (2020-06-20)
Axon regeneration is regulated by a neuron-intrinsic transcriptional program that is suppressed during development but that can be reactivated following peripheral nerve injury. Here we identify Prom1, which encodes the stem cell marker prominin-1, as a regulator of the axon
Protocols
This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.
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