ABF1052

Anti-Adenovirus 5 L4-33K

• Synonym(s): L4-33K, Protein 33K, Splicing factor 33K, Terminase small subunit
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• mol wt: calculated mol wt 25.16 kDa; observed mol wt ~35 kDa
• purified by: (Unpurified)
• species reactivity: virus
• packaging: antibody small pack of 100
• technique(s): immunofluorescence: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: Unknown
• Protein ID accession no.: AP_000205
• UniProt accession no.: P24940
• storage temp.: -10 to -25°C
• Gene Information: vaccinia virus ... L4(2653005)

Description

Specificity

This rabbit polyclonal antibody detects Protein 33K of Human Adenovirus 5.

Immunogen

His-tagged, recombinant fragment corresponding to 124 amino acids from the C-terminal half of human adenovirus 5.

Application

Quality Control Testing

Evaluated by Western Blotting in lysate from HeLa cells infected Human adenovirus type 5.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Protein 33K of Adenovirus 5 in lysate from HeLa cells infected with human adenovirus type 5, but not in lysate from uninfected cells.

Tested Applications

Western Blotting Analysis: A representative lot detected Adenovirus L4-33K in Western Blotting application. (Subramanian, T., et al. (2006). J. Virol. 80(4); 2000-2012).

Immunofluorescence Analysis: A representative lot detected Adenovirus L4-33K in Immunofluorescence application. (Charman, M., et al. (2023). Nature. 616(7956):332-338).

Western Blotting Analysis: A 1:2,500 dilution from a representative lot detected Adenovirus 5 L4-33K in HeLa cells infected with Human adenovirus type 5. (Courtesy of Dr. Patrick Hearing, Stony Brook University).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Protein 33K (UniProt: P24940; also known as L4-33K, Splicing factor 33K, Terminase, small subunit) is encoded by the L4 gene (Gene ID: 2653005) in human Adenovirus 5. Adenovirus 5. Adenovirus 5 belongs to the Adenoviridae family of non-enveloped viruses that are characterized by linear, double-stranded DNA genomes. Their capsid is an icosahedron that contains at least 12 different viral proteins. Adenovirus 5 is commonly used for gene transfer experiments due to its ability to infect a wide group of different cell types. It can harbor large genes in its genome incorporated via homologous recombination techniques. L4-33K is a homooligomeric protein that serves as a virus-encoded alternative RNA splicing factor. It is expressed in the late phase of viral replicative cycle. It activates splicing of viral late gene transcripts that contain weak 3 splice sites. It contains a short arginine-serine repeat region (aa 178-198) that is essential for its splicing enhancer function. L4-33K also plays a role in adenovirus assembly and in adenovirus morphogenesis by promoting the packaging of the viral genome into the empty capsid. It is essential for the temporal activation of major late pre-mRNA splicing at late stages of infection and it induces the splicing and expression of the late capsid vertex protein. It is suggested to function as the small terminase that is part of the molecular motor that translocates genomic DNA in empty capsid during DNA packaging. It is not detected in mature virions. The L4-33K protein may be phosphorylated by DNA-dependent protein kinase (DNA-PK) and protein kinase A (PKA). Its phosphorylation by DNA-PK is reported to have an inhibitory action in L4-33K-mediated alternative RNA splicing whereas the phosphorylation by PKA stimulates L4-33K-mediated alternative RNA splicing. Two isoforms of L4-33K have been described that are produced by alternative splicing. (Ref.: Charman, M., et al. (2023). Nature. 616(7956):332-338; Pérez-Berná, AJ., et al. (2014). J. Virol. 88(3); 1513-1524; Wu, K., et al. (2013). J. Virol. 87(12); 6739-6747).

Physical form

Rabbit polyclonal antisera without azide.

Reconstitution

Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable