ABE479

Anti-hMOF Antibody

from rabbit

• Synonym(s): Histone acetyltransferase KAT8, Lysine acetyltransferase 8, MOZ, YBF2/SAS3, SAS2 and TIP60 protein 1, MYST-1, hMOF
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: mouse, human, rat
• technique(s): ChIP: suitable; western blot: suitable
• NCBI accession no.: NP_115564
• UniProt accession no.: Q9H7Z6
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... KAT8(84148)

Description

General description

hMOF, also known as Histone acetyltransferase KAT8, or Lysine acetyltransferase, MOZ, YBF2/SAS3, SAS2, TIP60 protein 1, and MYST-1, and encoded by the gene KAT8/MOF/MYST1, is a histone acetyltransferase which is involved in transcriptional activation and also is a component of the MSL complex involved in acetylation of nucleosomal histone H4 producing specifically H4K16ac. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. hMOF also a component of many other MSL related complexes including the methyltransferase complexes and interacts with numerous other related proteins including BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MOF/KAT8, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. hMOF is localized to the nucleus and nuclear chromosomes.

Immunogen

Linear peptide corresponding to hMOF.

Application

Anti-hMOF antibody detects level of hMOF & has been published & validated for use in hMOF.

Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated hMOF from HeLa cell lysate (Wu, L., et al. (2011). Mol Cell. 43(1):132-144.).

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated hMOF from HeLa cell lysate (Smith, E. R., et al. (2005). Mol Cell Biol. 25(21):9175-9188.).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Quality

Evaluated by Western Blotting in HUVEC cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected hMOF in 10 µg of HUVEC cell lysate.

Target description

~60 kDa observed. Uncharacterized band(s) may be observed in some cell lysate.

Physical form

Format: Purified

Protein G Purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable