Recognizes rat Epithelial Sodium Channel beta (ENaC beta). The immunogen shows no significant sequence homology with other ENaC subunits.
Immunogen
A 20 amino acid peptide sequence near the N-terminus of rat ENaC beta. The immunogen sequence is 100% conserved in mouse, 94% in rabbit, 81% in human, 76% in ovine and 64% in xenopus.
Application
Research Category Neuroscience
Research Sub Category Ion Channels & Transporters
Neuroinflammation & Pain
This Anti-Epithelial Sodium Channel-β Antibody is validated for use in ELISA, WB for the detection of Epithelial Sodium Channel-β.
Western blot: 1-10 μg/mL using ECL. 83-90kDa bands, membrane preparations are recommended (Masilamani et al., 1999).
ELISA: 0.5-1.0 mg/mL using 1 mg/mL control peptide to coat plate.
Optimal working dilutions must be determined by the end user.
Physical form
Affinity purified immunoglobulin in PBS containing 0.1% BSA.
Storage and Stability
Maintain at -20°C in undiluted aliquots for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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The shift of cytokine profile from anti- to pro-inflammatory is the most recognizable sign of labor, although the underlying mechanism remains elusive. Here, we report that the epithelial sodium channel (ENaC) is upregulated and activated in the uterus at labor
We wished to determine the effect of continuous β-receptor stimulation on alveolar fluid clearance and to elucidate the mechanisms behind this effect. Alveolar fluid clearance was measured in anaesthetized rats pretreated for 72 h with the β-agonist isoproterenol (200 μg kg(-1) h(-1) sc)
American journal of physiology. Renal physiology, 315(3), F653-F664 (2018-05-24)
The chronic intrinsic diuretic and natriuretic tone of sodium-glucose cotransporter 2 (SGLT2) inhibitors is incompletely understood because their effect on body fluid volume (BFV) has not been fully evaluated and because they often increase food and fluid intake at the
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