676472

VEGF₁₆₅, Human, Recombinant, S. frugiperda

• Synonym(s): Vascular Endothelial Growth Factor₁₆₅, rhVEGF, rhVPF, rh-Vasculotropin
• UNSPSC Code: 12352202

Properties

• Assay: ≥97% (SDS-PAGE)
• Quality Level: 100
• form: lyophilized
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• impurities: ≤1 EU/μg Endotoxin (EU/μg VEGF)
• shipped in: ambient
• storage temp.: −70°C

Description

General description

Recombinant, human VEGF₁₆₅ (165 amino acid splice variant of VEGF) expressed in S. frugiperda insect cells. A heparin-binding glycoprotein with potent angiogenic, endothelial cell-specific mitogenic and vascular permeability-enhancing activities. Increases endothelial cell proliferation after vascular injury. Supplied as a glycosylated, disulfied-linked homodimer.

Recombinant, human VEGF₁₆₅ (165 amino acid splice variant of VEGF) expressed in S. frugiperda insect cells. A heparin-binding glycoprotein with potent angiogenic, endothelial cell-specific mitogenic, and vascular permeability-enhancing activities. Increases endothelial cell proliferation after vascular injury. Supplied as a glycosylated, disulfide-linked homodimer.

Biochem/physiol Actions

ED₅₀ = 2.0-6.0 ng/ml as measured by its ability to stimulate ³H-thymidine incorporation into human umbilical vein endothelial cells

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from sterile-filtered 30% acetonitrile, 0.1% TFA, 50 µg BSA/µg VEGF.

Reconstitution

Following reconstitution, aliquot and freeze (-70°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -70°C.

Reconstitute to a final concentration of ≥1 µg/ml with sterile PBS containing ≥0.1% HSA or BSA.

Other Notes

Liu, X., et al. 2001. J. Urology166, 354.
Burke, P.A., et al. 1995. Biochem. Biophys. Res. Commun. 207, 348.
Neufeld, G., et al. 1994. Prog. Growth Factor Res.5, 89.
Leung, D.W., et al. 1989. Science 246, 1306.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3