574799

SynaptoRed^™ Reagent

A lipophilic, water-soluble styrylpyridinium red fluoroprobe that is useful for staining active recycling synaptic vesicles.

• Synonym(s): SynaptoRed^™ Reagent
• Empirical Formula (Hill Notation): C₃₀H₄₅Br₂N₃
• CAS Number: 162112-35-8
• Molecular Weight: 607.51
• MDL number: MFCD00467819
• UNSPSC Code: 12171500
• NACRES: NA.25

Properties

• Quality Level: 100
• Assay: ≥95% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic); protect from light
• color: dark purple
• solubility: water: 10 mg/mL; DMSO: soluble; methanol: soluble
• shipped in: ambient
• storage temp.: −20°C
• SMILES string: [Br-].[Br-].[N+](CCC[n+]1ccc(cc1)C=CC=CC=Cc2ccc(cc2)N(CC)CC)(CC)(CC)CC
• InChI: 1S/C30H45N3.2BrH/c1-6-32(7-2)30-20-18-28(19-21-30)16-13-11-12-14-17-29-22-25-31(26-23-29)24-15-27-33(8-3,9-4)10-5;;/h11-14,16-23,25-26H,6-10,15,24,27H2,1-5H3;2*1H/q+2;;/p-2
• InChI key: AFVSZGYRRUMOFH-UHFFFAOYSA-L

Description

General description

A lipophilic, nontoxic, water-soluble styrylpyridinium red fluoroprobe that is useful for staining active recycling synaptic vesicles. Also useful for studying endocytosis, membrane turnover, and identifying cell membrane boundaries. This compound is non-fluorescent in aqueous solution, but becomes fluorescent when incorporated into the plasma membrane. Reversible, photostable material that does not diffuse out of internalized vesicles. Can also be used in combination with the SynaptoGreen^™ (Cat. No. 574798) to image plasma membranes independently in two colors. Excitation max.: 538-548 nm; emission max.: ~735 nm.

A lipophilic, water-soluble styrylpyridinium red fluoroprobe that is useful for staining active recycling synaptic vesicles.

A lipophilic, water-soluble styrylpyridinium red fluoroprobe that is useful for staining active recycling synaptic vesicles. Also useful in studying endocytosis and identifying cell membrane boundaries. The compound is non-fluorescent in aqueous solution but becomes fluorescent when incorporated into the plasma membrane. Reversible, photostable, and does not diffuse out of internalized vesicles. Can also be used in combination with other green fluoroprobes for double-labeling experiments.

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Durand, J., et al. 2000. Neuroscience97, 11.
Rouze, N.C. and Schwartz, E.A. 1998. J. Neurosci.18, 8614.
Vida, T.A. and Emr, S.D. 1995. J. Cell Biol.128, 779.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

SynaptoGreen is a trademark of Biotium, Inc.

SynaptoRed is a trademark of Biotium, Inc.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable