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539229

Sigma-Aldrich

Anti-Protein Disulfide Isomerase Rabbit pAb

liquid, Calbiochem®

Synonym(s):

Anti-PDI

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≥0.9% sodium azide as preservative

species reactivity

guinea pig, Xenopus, sheep, bovine, rat, canine, hamster, porcine, mouse, monkey, human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PDIA2(64714)

General description

Protein A purified rabbit polyclonal antibody. Recognizes the ~55 kDa (apparent MW) protein disulfide isomerase protein.

Immunogen

Bovine
bovine liver Protein Disulfide Isomerase

Application

Immunoblotting (1:1000, colorimetric)

Immunocytochemistry (see comments)

Immunoprecipitation (see comments)

Paraffin Sections (see comments)

Warning

Toxicity: Standard Handling (A)

Physical form

In PBS, 50% glycerol.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
HeLa cells, rat testis, mouse spleen

Other Notes

Cribb, A.E., et al. 1997. J. Pharmacol. Exp. Ther.282, 1064.
Freedman, R.B., and Hillson, D.A. 1980. In Enzymology of post-translational modification of proteins. (Freedman R.B. and Hawkin H.C. eds.) Academic, London p. 157.
This antibody will also work in immunocytochemistry, immunoprecipitation, and paraffin sections; the concentration must be determined for each sample analyzed. Variables associated with assay conditions will dictate the optimal working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Lihe Zhang et al.
Metabolism: clinical and experimental, 57(8), 1115-1124 (2008-07-22)
High fructose consumption is associated with the development of fatty liver and dyslipidemia with poorly understood mechanisms. We used a matrix-assisted laser desorption/ionization-based proteomics approach to define the molecular events that link high fructose consumption to fatty liver in hamsters.
Alvaro Souto Padron de Figueiredo et al.
The Journal of biological chemistry, 290(21), 13427-13439 (2015-04-01)
Inflammation and oxidative stress through the production of reactive oxygen species (ROS) are consistently associated with metabolic syndrome/type 2 diabetes. Although the role of Nox2, a major ROS-generating enzyme, is well described in host defense and inflammation, little is known
Anthony O Gramolini et al.
Molecular & cellular proteomics : MCP, 7(3), 519-533 (2007-12-07)
Defective mobilization of Ca2+ by cardiomyocytes can lead to cardiac insufficiency, but the causative mechanisms leading to congestive heart failure (HF) remain unclear. In the present study we performed exhaustive global proteomics surveys of cardiac ventricle isolated from a mouse

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